补肾益冲方对卵巢储备功能减退大鼠卵巢组织形态及性激素的影响  

The effect of Bushen Yichong formula on ovarian tissue morphology and sex hormones in rats with diminished ovarian reserve

作　　者：简晓莉[1,2] 刘志承 徐汉明[3] 常瑞萍[1] Jian Xiaoli;Liu Zhicheng;Xu Hangming;Chang Ruiping(Guangzhou University of Traditional Chinese Medicine Fourth Clinical Medical College Shenzhen Traditional Chinese Medicine Hospital,Guangdong518033;Key Laboratory of Traditional Chinese Medicine Formulations in Shenzhen Hospitals,Guangdong518033;Peking University Shenzhen Hospital,Guangdong 518033)

机构地区：[1]广州中医药大学第四临床医学院深圳市中医院,广东518033 [2]深圳市医院中药制剂研究重点实验室,广东518033 [3]北京大学深圳医院,广东518033

出　　处：《天津药学》2024年第1期1-4,共4页Tianjin Pharmacy

基　　金：广东省中医药局项目(No.20221353);深圳市医疗卫生三名工程项目(No.SZZYSM202111002)。

摘　　要：目的:研究补肾益冲方对卵巢储备功能减退(DOR)模型大鼠卵巢功能的影响。方法:选取雌性未孕SD大鼠40只,随机分为空白组、模型组、补肾益冲方组、补佳乐组。除空白组外,其余大鼠采用雷公藤多苷片混悬液灌胃制备DOR大鼠模型,造模成功后分别给予补肾益冲方及补佳乐进行相应的治疗干预。药物干预结束后,采用酶联免疫吸附试验法(ELISA)检测大鼠血清中抗苗勒管激素(AMH)、促卵泡生成素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)的水平;HE染色观察大鼠卵巢组织的病理学形态变化。结果:与空白组比较,模型组大鼠血清FSH和LH水平升高(P<0.05),AMH和E_(2)水平降低(P<0.05);卵巢中原始卵泡和生长卵泡的数量减少,闭锁卵泡增加;卵巢间质增生并纤维化。补肾益冲方干预后,DOR大鼠血清中FSH和LH激素水平降低(P<0.05),AMH和E_(2)激素水平升高(P<0.05);卵巢组织形态得到改善,原始卵泡和生长卵泡增多,闭锁卵泡减少。结论:补肾益冲方对DOR大鼠卵巢组织形态、卵母细胞的数量和质量具有改善作用,能调节AMH、FSH、E_(2)和LH从而提高DOR大鼠的卵巢储备功能。Objective:To investigate the effect of Bushen Yichong formula on ovarian reserve function in rats with a diminished ovarian reserve(DOR)model.Methods:Forty female non-pregnant SD rats were selected and randomly divided into blank group,model group,Bushen Yichong formula group,and Premarin group.Except for the blank group,the remaining rats were treated with tripterysium polyglycoside tablets to prepare a DOR rat model.After successful modeling,the rats received corresponding therapeutic interventions.After the drug intervention,the serum levels of anti-mullerian hormone(AMH),follicle-stimulating hormone(FSH),estradiol(E_(2)),and luteinizing hormone(LH)were measured by enzyme-linked immunosorbent assay(ELISA),and the pathological changes in the ovarian tissues of the rats were observed by hematoxylin-eosin(HE)staining.Results:Compared with the blank group,the serum levels of FSH and LH in the model group rats were increased(P<0.05),and the levels of AMH and E_(2)were decreased(P<0.05);the number of primordial follicles and growing follicles in the ovaries was decreased,and atretic follicles were increased;The interstitium of the ovaries was hyperplasia and fibrosis.After the intervention of Bushen Yichong formula,the serum levels of FSH and LH hormones in DOR rats decreased(P<0.05),and the levels of AMH and E_(2)hormones increased(P<0.05);the morphology of ovarian tissues was improved,with an increase in the number of primordial follicles and growing follicles,and a decrease in the number of atretic follicles.Conclusion:Bushen Yichong formula has an improving effect on the morphology and quality of ovarian tissue in DOR rats and can regulate AMH,FSH,E_(2),and LH levels to improve the ovarian function of DOR rats.

关 键 词：补肾益冲方 卵巢储备功能减退 性激素 卵泡 

分 类 号：R285.5[医药卫生—中药学]