虎黄烧伤搽剂中白藜芦醇苷对皮肤癌大鼠放疗后细胞凋亡、免疫功能和JNK信号通路的影响  

Effect of Polydatin in Huhuang Burn Limiment(虎黄烧伤搽剂)on Apoptosis,Immune Function and JNK Signaling Pathway in Rats with Skin Cancer after Radiotherapy

作　　者：肖明伦 吴红霞 沙前坤 陈瑾[3] 胡志帮 XIAO Mingun;WU Hongxia;SHA Qiankun;CHEN Jin;HU Zhibang(People's Hospital of Chongqing Banan District,Chongqing 401320,China;Pharmacy Department,Chongqing Yangdu Biology Institute,Chongqing 408000,China;The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China:4.Affiliated Hospital of Chongqing Three Gorges Medical College,Chongqing 404000,China;Affiliated Hospital of Chongqing Three Gorges Medical College,Chongqing 404000,China)

机构地区：[1]重庆医科大学附属巴南医院,重庆401320 [2]重庆央都生物研究院药学部,重庆408000 [3]重庆医科大学附属第一医院,重庆400016 [4]重庆三峡医药高等专科学校附属医院,重庆404000

出　　处：《中华中医药学刊》2024年第1期66-69,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81372917);重庆市自然科学基金面上项目(csct2021jcyj-msxX0718)。

摘　　要：目的探究虎黄烧伤搽剂中白藜芦醇苷对皮肤癌大鼠放疗后细胞凋亡、免疫功能和JNK信号通路的影响。方法将80只SD大鼠分为对照组和模型组,对照组20只,模型组60只;模型组进行大鼠皮肤癌模型建立,并使用60Gy剂量射线照射癌变部位,之后分别用10、20、30 mg/kg的虎黄烧伤搽剂白藜芦醇苷对模型组大鼠进行灌胃处理,分为10 mg/kg组、20 mg/kg组、30 mg/kg组。流式细胞术检测细胞凋亡情况;酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测大鼠免疫功能;蛋白质印迹(Western blot)检测应激活化蛋白酶(c-Jun N-terminal protein kainse,JNK)、磷酸化的JNK(phosphorized JNK,p-JNK)、半胱氨酸天冬氨酸蛋白水解酶-3(Caspase-3)、B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)和B淋巴细胞瘤-2相关蛋白(Bcl-2 associated protein,Bax)表达情况。结果与对照组相比,模型组细胞凋亡率显著降低(P<0.05);Bcl-2蛋白表达显著增加(P<0.05);Bax蛋白表达显著降低(P<0.05)。与模型组相比,10、20、30 mg/kg虎黄烧伤搽剂白藜芦醇苷显著增加了细胞凋亡率(P<0.05);显著降低了Bcl-2蛋白表达(P<0.05);显著增加了Bax蛋白表达(P<0.05)。与对照组相比,模型组血清白细胞介素-β(Interleukin-β,IL-β)、白细胞介素-6(Interleukiin-6,IL-6)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)炎症因子水平显著升高(P<0.05);丙二醛(Malonaldehyde,MDA)、肌酸激酶(Creatine Kinase,CK)、肌钙蛋白I(TroponinI,TnI)活性显著升高(P<0.05);SOD活性显著降低(P<0.05)。与模型组相比,10、20、30 mg/kg虎黄烧伤搽剂白藜芦醇苷显著降低了血清IL-β、IL-6、TNF-α炎症因子水平(P<0.05);MDA、CK、TnI活性显著降低(P<0.05);超氧化物歧化酶(Superoxide dismutase,SOD)活性显著升高(P<0.05)。与对照组相比,模型组JNK蛋白水平差异无统计学意义(P>0.05);p-JNK蛋白水平显著升高(P<0.05)。与模型组相比,10、20、30 mg/kg虎黄烧伤搽剂白藜芦醇苷对JNK蛋�Objective To investigate the effects of resveratrol glycosides in Huhuang Burn Limiment(虎黄烧伤搽剂)on apoptosis,immune function and JNK signaling pathway in skin cancer rats after radiotherapy.Methods Eighty SD rats were divided into a control group and a model group,20 in the control group and 60 in the model group.The the rat skin cancer model was established in the model group and the cancerous site was irradiated with 60Gy dose radiation.The resveratrol glycosides in the Huhuang Burn Limiment(10 mg/kg,20 mg/kg and 30 mg/kg)were administered orally to rats in the model group,which were divided into 10 mg/kg group,20 mg/kg group,30 mg/kg group.Flow cytometry was used to detect the apoptosis.Enzyme-linked immunosorbent assay(ELISA)was used to detect the immune function of the rats.Western blot was used to detect activated proteinase(JNK),phosphorylated JNK(p-JNK)and cystine aspartic acid protease-3(Caspase-3),B lymphoma-2(Bcl-2)and B lymphoma-2 related protein(Bax).Results Compared with those of the control group,the apoptosis rate in the model group was significantly reduced(P<0.05),the expression of Bcl-2 protein was significantly increased(P<0.05)and the expression of Bax protein was significantly reduced(P<0.05).Compared with those of the model group,the resveratrol glycosides in Huhuang Burn Limiment groups(10 mg/kg,20 mg/kg and 30 mg/kg)significantly increased the apoptosis rate(P<0.05),significantly reduced Bcl-2 protein expression(P<0.05)and Bax protein expression was significantly increased(P<0.05).Compared with those of the control group,the serum levels of IL-β,IL-6 and TNF-α in the model group were significantly increased(P<0.05),the activities of MDA,CK and TnI were significantly increased(P<0.05)and the activity of SOD was significantly reduced(P<0.05).Compared with those of the model group,resveratrol glycosides Huhuang Burn Limiment groups(10 mg/kg,20 mg/kg and 30 mg/kg)significantly reduced serum levels of IL-β,IL-6 and TNF-α(P<0.05).The activities of MDA,CK and TnI were significantl

关 键 词：虎黄烧伤搽剂 白藜芦醇苷 皮肤癌大鼠 细胞凋亡 免疫功能 JNK信号通路 

分 类 号：R289.5[医药卫生—方剂学] R282.71[医药卫生—中药学]