酶解血粉制备氨基酸水溶肥工艺条件优化  

Optimization of process conditions for preparation of amino acid water-soluble fertilize by enzymatic hydrolysis of blood powder

作　　者：张洪志 沈涛[1] 杜新园 杨育乾 ZHANG Hongzhi;SHEN Tao;DU Xinyuan;YANG Yuqian(Ningxia Xiasheng Industrial Group Co.,Ltd.,Yinchuan 750000,China)

机构地区：[1]宁夏夏盛实业集团有限公司,宁夏银川750000

出　　处：《磷肥与复肥》2024年第1期11-15,共5页Phosphate & Compound Fertilizer

摘　　要：为资源化利用动物血液,以血粉为原料制备氨基酸水溶肥。通过单因素实验研究液料比、原料预处理温度、蛋白酶种类及组合酶比例等因素对血粉蛋白酶解效果的影响;通过正交实验考察温度、酶制剂添加量、p H和时间对血粉蛋白酶解效果的影响。结果表明:血粉的最佳酶解工艺为液料体积质量比6 mL/g、温度55℃、组合酶添加量3.0%(组合酶中蛋白酶B与风味蛋白酶质量比为2∶1)、pH 8.0、时间12 h;在该工艺条件下,血粉蛋白水解度为67.26%,酶解液中19种游离氨基酸质量分数为6.39%,分子量在1046 Da以下的多肽占比达到99.59%。通过对血粉酶解工艺的优化,提高了血粉蛋白的水解度,增加酶解液中氨基酸含量,为蛋白酶酶解血液制备氨基酸水溶肥的过程控制及规模应用提供理论指导。In order to utilize animal blood resources,amino acid water-soluble fertilizer is prepared using blood powder as raw material.The effects of factors such as liquid material ratio,raw material pretreatment temperature and combination enzyme ratio on the efficiency of blood powder enzymatic hydrolysis are studied through single factor experiments;The effects of temperature,enzyme dosage,pH and time on the efficiency of blood powder enzymatic hydrolysis are investigated through orthogonal experiments.The results show that the optimal enzymatic hydrolysis process for blood powder are as follows:The ratio of liquid volume to material mass is 6 mL/g,the temperature is 55℃,the addition amount of combined enzyme is 3.0%(the mass ratio of protease B to flavor protease in the combined enzyme is 2:1),the pH is 8.0,and the time is 12 hours.Under these process conditions,the hydrolysis degree of blood powder protein is 67.26%,the mass fraction of 19 free amino acids in the enzymatic solution is 6.39%,and the proportion of peptides with molecular weight below 1046 Da reaches 99.59%.By optimizing the enzymatic hydrolysis process of blood powder,the hydrolysis degree of blood powder protein has been improved,and the amino acid content in the enzymatic hydrolysis solution has been increased,providing theoretical guidance for the process control and large-scale application of enzymatic hydrolysis of blood to prepare amino acid water-soluble fertilizer.

关 键 词：血粉 蛋白酶 氨基酸水溶肥 工艺条件 优化 

分 类 号：S143.8[农业科学—肥料学] TS251.93[农业科学—农业基础科学]