Sysmex UF-5000全自动尿有形成分分析仪细菌计数和尿液细菌培养一致性评价  

Consistency evaluation of Sysmex UF-5000 urinary bacterial count and urinary bacterial culture methods

作　　者：张爱爱 罗蓉 Zhang Aiai;Luo Rong(Department of Medical Laboratory,Fenyang Hospital of Shanxi Province,Fenyang 032200,China)

机构地区：[1]山西省汾阳医院医学检验科,汾阳032200

出　　处：《实用医技杂志》2024年第2期108-111,I0002,共5页Journal of Practical Medical Techniques

摘　　要：目的评价UF-5000全自动尿有形成分分析仪细菌计数与尿细菌培养结果的一致性,探讨Sysmex UF-5000全自动尿有形成分分析仪细菌计数在诊断尿路感染中的应用价值。方法采集山西省汾阳医院2022年8月至2023年1月门诊及住院患者清洁中段尿标本192例,样本首先进行尿细菌培养及鉴定,然后用UF-5000尿有形成分分析仪对尿液中细菌进行计数及分类。以尿液细菌培养和鉴定的结果为诊断的金标准,将样本分为阳性组和阴性组,分别对2组的细菌计数结果进行统计分析,然后利用统计结果绘制出受试者工作特征曲线(ROC曲线),以此得到最佳的细菌计数诊断阈值(cut-off值)和曲线下面积(AUC),从而对尿细菌计数和尿细菌培养两种方法的检验结果一致性进行评价。结果192例标本中有120例阳性(62.5%)和72例阴性(37.5%)。根据绘制出的ROC曲线结果得出,曲线下面积(AUC)为0.937,对应诊断阈值为312.00CFU/ml,此时灵敏度为87.5%,特异度为90.3%。结论尿细菌计数可作为快速筛查尿路感染患者,其与尿细菌培养方法的一致性评价较好,有较高的诊断价值。但其准确性仍有待提高,并不可完全取代尿细菌培养。Objective To evaluate the consistency between the bacterial count of Sysmex UF-5000 full-automatic urine formed fraction analyzer and the results of urine bacterial culture,and to explore the application value of bacterial count of Sysmex UF-5000 full-automatic urine formed fraction analyzer in the diagnosis of urinary tract infection.Methods A total of 192 clean mid-stage urine specimens were collected from outpatients and inpatients of Fenyang Hospital,Shanxi Province,from August 2022 to January 2023.The samples were firstly subjected to urine bacterial culture and identification,and then counted and categorized by the UF-5000 full-automatic urine formed fraction analyzer.Taking the results of urine bacterial culture and identification as the gold standard for diagnosis,the samples were divided into positive and negative groups,and the bacterial counting results of the two groups were analyzed statistically,and then the statistical results were used to draw the receiver operating characteristic curve(ROC curve)of the subjects,so as to get the optimal bacterial counting diagnostic threshold(cut-off value)and the area under the curve(AUC),and then the consistency of the test results of the two methods of urine bacterial counting and urine bacterial culture was.Results There were 120 positive(62.5%)and 72 negative(37.5%)cases out of 192 specimens.According to the ROC curve,the area under the curve(AUC)was 0.937,which corresponded to a diagnostic threshold of 312.00 CFU/ml,with a sensitivity of 87.5%and a specificity of 90.3%.Conclusion Urine bacterial count can be used as a rapid screening for patients with urinary tract infections,and its consistency with urine bacterial culture methods is evaluated better,with higher diagnostic value.However,its accuracy still needs to be improved and cannot completely replace urine bacterial culture.

关 键 词：尿 细菌载量 细菌培养 ROC曲线 

分 类 号：R446.12[医药卫生—诊断学]