过度劳累对小鼠脾脏造血功能的影响  

Effect of overwork on hemopoietic function of spleen in mice

作　　者：邓三春 陈苏衡 于恺华 郭鸿 廖阅 鲍振星 李玉兰[2] DENG Sanchun;CHEN Suheng;YU Kaihua;GUO Hong;LIAO Yue;BAO Zhenxing;LI Yulan(The First Clinical Medical College,the First Hospital of Lanzhou University,Lanzhou University,Lanzhou,Gansu Province,730000,China;Department of Anesthesiology,the First Hospital of Lanzhou University,Lanzhou University,Lanzhou,Gansu Province,730000,China)

机构地区：[1]兰州大学第一临床医学院,兰州730000 [2]兰州大学第一医院麻醉科,兰州730000

出　　处：《陆军军医大学学报》2024年第5期427-433,共7页Journal of Army Medical University

基　　金：甘肃省卫生健康行业科研项目(GSWSKY2021-079)。

摘　　要：目的探讨过度劳累(过劳)对小鼠脾脏造血功能的影响。方法C57BL/6J小鼠18只,按随机数字表法分为3组(n=6):对照(C)组、过劳15 d(W1)组、过劳30 d(W2)组。W1、W2组每日强迫水中站立8 h+束缚3 h,分别持续15、30 d,C组无特殊处理。观察测量小鼠一般情况、进食量和体质量,造模结束后取血、处死小鼠,分离脾脏和心脏,测量脾脏指数;检测血常规;脾脏行HE染色;采用RT-qPCR法检测小鼠脾脏趋化因子CXC配体12(C-X-C motif chemokin ligand 12,CXCL12)、干细胞因子(stem cell factor,SCF)和心脏间隙连接蛋白45(connexin 45,Cx45)mRNA表达水平;采用ELISA检测脾脏CXCL12、SCF蛋白表达水平;免疫组化法行心脏Cx45蛋白定量。结果①一般情况:与C组相比,W1组、W2组小鼠毛发粗糙,活跃程度先增加后下降,扎堆、精神状态差;小鼠进食量:W1、W2组显著高于同时间C组;C组小鼠体质量随喂养时间增加而增加,W1、W2组体质量无明显增长;W2组小鼠脾脏指数显著下降(P<0.05);②血常规:与C组相比,W2组WBC、RBC数目、HGB浓度显著升高(P<0.05);W1组、W2组PLT数目显著升高(P<0.05);③脾脏HE染色:与C组相比,W2组小鼠脾脏红髓与白髓分界欠清,红髓区域增大;④RT-qPCR检测结果显示:W2组心脏组织Cx45 mRNA表达显著高于C组(P<0.05);脾脏组织SCF mRNA表达显著高于C组和W1组(P<0.05);⑤免疫组化检测结果显示:W1组和W2组小鼠心肌Cx45蛋白含量显著高于C组(P<0.05);⑥ELISA检测结果显示:W2组脾脏组织SCF蛋白表达显著高于C组和W1组(P<0.05),W1组较C组差异无统计学意义。结论过劳可破坏心肌细胞间的紧密连接,增强脾脏造血功能,使全血细胞数目过度增加,形成血液高凝状态,这可能是过劳死相关心血管疾病的诱发因素之一。Objective To investigate the effect of overwork on the hemopoietic function of spleen in mice.Methods A total of 18 C57BL/6J mice were randomly divided into 3 groups,i.e.,control group(C),15-day-overworked group(W1)and 30-day-overworked group(W2).The mice from Groups W1 and W2 were forced to keep standing in water for 8 h and restrained for 3 h for consecutive 15 and 30 d,respectively,while those of Group C had no special treatment.The general condition,food intake and body weight of the mice were observed and measured.After modelling,the mice were euthanatized,and the spleen and heart were isolated to calculate spleen index and observe spleen pathological changes after HE staining,and then blood routine test was performed.The mRNA expression levels of CXCL12 and SCF in the spleen and that of connexin 45(Cx45)in the heart were detected by RT-qPCR,and the protein levels of CXCL12 and SCF in spleen were detected by ELISA.The cardiac protein level of Cx45 was quantified with immunohistochemical assay.Results①General condition showed that Groups W1 and W2 showed coarse hairs,increased at first and then decreased in activities,poor mental state,and larger food intake when compared with Group C.The body weight of Group C was increased with the feeding time,but that of Groups W1 and W2 had no such change.The spleen index was decreased significantly in Group W2(P<0.05).②The results of blood routine test showed that WBC and RBC counts and HGB level were significantly increased in Group W2(P<0.05),and PLT count in Groups W1 and W2 was significantly increased compared with Group C(P<0.05).③Spleen HE staining displayed that the boundary between red pulp and white pulp was not clear,and the region of red pulp was enlarged in Group W2 when compared with Group C.④RT-qPCR showed that the mRNA level of Cx45 was significantly higher in heart tissue of Group W2 than that of Group C(P<0.05),and that of SCF in spleen tissue of Group W2 was also higher than that in Groups C and W1(P<0.05).⑤Immunohistochemical assay indicated

关 键 词：过度劳累 髓外造血 脾脏 心肌梗死 

分 类 号：R331.21[医药卫生—人体生理学] R363.22[医药卫生—基础医学] R873