不同栽培模式对山丹丹根际土壤细菌群落多样性的影响研究  

Studied on bacterial diversity of rhizosphere soil from different cultivated Lilium pumilum

作　　者：李静[1] 郑毅鹏 郭旻皓 王丹[1] 齐向英[1] 邓振山[1] LI Jing;ZHENG Yipeng;GUO Minghao;WANG Dan;QI Xiangying;DENG Zhenshan(College of Life Sciences,Yan’an University,Yan’an 716000,China;College of Life Sciences,Northwest University,Xi’an 710069,China)

机构地区：[1]延安大学生命科学学院,延安716000 [2]西北大学生命科学学院,西安710069

出　　处：《生态科学》2023年第6期134-143,共10页Ecological Science

基　　金：陕西省县域重点科技项目(2018XY—14);2019年延安大学校级大学生创新创业训练计划项目(D2019114);陕西省重大科技专项(2022ZDLNY03—10)。

摘　　要：采集陕西子长市高柏山示范基地山丹丹根际土壤,利用高通量测序技术,分析其细菌群落的多样性及其影响因素。结果表明:共获得细菌有效序列575510条,包含30门90纲147目242科391属。优势菌门为放线菌门(Actinobacteria,38.56%—46.12%)、变形菌门(Proteobacteria,16.67%—26.55%)、绿弯菌门(Chloroflexi,9.45%—18.57%)、酸杆菌门(Acidobacteria,2.96%—5.26%)和拟杆菌门(Bacteroidetes,2.04%—5.76%)。优势菌纲为放线菌纲(Actinobacteria,32.44%—38.52%)、α-变形菌纲(Alphaproteobacteria,15.47%—25.89%)、酸杆菌纲(Acidimicrobiia,2.39%—7.59%)、热微菌纲(Thermomicrobia,3.43%—5.55%)和氯酸杆菌纲(Chloracidobacteria,2.14%—4.29%)。冗余分析显示在属水平上,环境因子可以解释61.86%的细菌群落差异。有机质、速效钾和有效磷是影响山丹丹根际土壤细菌群落组成产生差异的主要环境因素。研究表明,山丹丹根际土壤细菌群落多样性丰富,栽培模式会影响山丹丹根际土壤细菌群落组成产生差异。该研究可为山丹丹菌种资源的进一步开发提供参考。In order to reveal the diversity and influencing factors of soil bacterial community,it takes the rhizosphere soil of Lilium pumilum DC.in Gaobai Mountain demonstration Base in Zichang City,Shaanxi Province as the main research material.High-throughput sequencing technique was used to determine the bacterial community in different soil collected.The results showed that a total of 575510 effective sequences of bacteria were obtained by culture-free method,including 30 phyla,90 classes,147 orders,242 families and 391 genera.The dominant bacteria phyla were Actinobacteria(38.56%-46.12%),Proteobacteria(16.67%-26.55%)and Chloroflexi(9.45%-18.57%),Acidobacteria(2.96%-5.26%)and Bacteroidetes(2.04%-5.76%).The dominant bacteria classes were Alphaproteobacteria(32.44%-38.52%),α-Metamorphosis(15.47%-25.89%),Acidimicrobiia(2.39%-7.59%),Thermomicrobia(3.43%-5.55%)and Chlorobacter(2.14%-4.29%).Redundancy analysis showed that environmental factors could explain 61.86%of the changes in bacterial communities at the genus level.Organic matter,available potassium and available phosphorus were the main factors affecting the diversity of bacterial community composition in the rhizosphere soil of Lilium pumilum.The results showed that the diversity of bacterial community in the rhizosphere soil of Lilium pumilum was rich,and the cultivation pattern would affect the difference of bacterial community composition in Lilium pumilum rhizosphere soil.This study can provide reference for the further development of Lilium pumilum strain resources.

关 键 词：山丹丹 根际土壤 细菌多样性 高通量测序 

分 类 号：Q938.1[生物学—微生物学]