多波段LED促进皮肤创伤修复的研究  

The Promotion of Multiband-LED in Wound Healing of Cutaneous Cells

作　　者：米晶[1] 吴珊 Mi Jing;Wu Shan(Department of Plastic Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海200011

出　　处：《应用激光》2024年第1期161-166,共6页Applied Laser

摘　　要：为明确多波段发光二极管(LED)促进皮肤表皮角质形成细胞和成纤维细胞创伤修复的作用,通过机械损伤分别建立体外培养的人皮肤角质形成细胞、皮肤成纤维细胞创伤模型,采用含460 nm、590 nm、640 nm及940 nm波峰的多波段LED光分别照射损伤角质形成细胞(HaCaT)和成纤维细胞(HS27)后,评估角质形成细胞的增殖和迁移速度、成纤维细胞释放炎症因子及产生细胞外基质的水平。结果显示,多波段LED光产生的低能量光治疗(low level light therapy,LLLT)可以促进损伤后皮肤角质形成细胞增殖和迁移,减少损伤后皮肤成纤维细胞释放的白介素-6(interlukin-6,IL-6)和肿瘤坏死因子-α水平(Tumor necrosis factor,TNF-α),促进损伤修复后皮肤成纤维细胞生成细胞外基质(extracellular matrix,ECM),且调节皮肤细胞损伤修复的作用强度与光疗次数呈量-效关系。研究表明,多波段LED光介导的光生物调节作用(photobiomodulation,PBM)可通过加速表皮细胞再上皮化、抑制真皮成纤维细胞炎症反应以及诱导ECM产生,促进皮肤损伤修复。OBJECTIVE To investigate the effect of multiband-light-emitting diode(LED) in regulating cutaneous cells' functions after skin injury.METHOD The epidermal keratinocytes(HaCaT) and dermal fibroblasts(HS27) of human were injured by mechanical injury,respectively.The injured cells were treated by a LED light that with four peak bands(460 nm,590 nm,640 nm,and 940 nm) and output energy density of 400 mW/cm^(2).The proliferation and migration of HaCaT cells were evaluated by CTG assay and wound healing assay,respectively.Moreover,the proinflammatory factors in HS27,such as interlukin-6(IL-6) and tumor necrosis factor-α(TNF-α),were detected by ELISA.In addition,the extracellular matrix(ECM) proteins production of HS27 were evaluated by western blotting and ELISA.RESULTS Low level light therapy(LLLT) with the multiband-LED i) accelerated cell proliferation and migration of injured keratinocytes,ii) decreased the protein levels of IL-6 and TNF-α in the injured fibroblasts,iii) increased the production of Collagen-I,Collagen-III,and hyaluronic acid.Furthermore,a better improved wound healing effect were seen after two treatments,compared with that after only one treatment.CONCLUSION The multiband-LED mediated photobiomodulation accelerated re-epithelialization,ameliorated dermal inflammation and stimulated ECM production,promoting wound healing of skin.

关 键 词：多波段LED 低能量光疗 光生物调节作用 创伤修复 

分 类 号：O436[机械工程—光学工程]