利用单壁碳纳米管介导植物瞬时遗传转化的研究  

Use of single-walled carbon nanotubes to mediate transient genetic transformation in plants

作　　者：刘晶慧 姚晓青 罗军玲[1] 吴刚[1] 闫晓红[1] LIU Jing-hui;YAO Xiao-qing;LUO Jun-ling;WU Gang;YAN Xiao-hong(Oil Crops Research Institute of Chinese Academy of Agricultural Science/Supervision and Test Center(Wuhan)for Plant Ecological Environment Safety,Ministry of Agriculture and Rural Affairs/Key Laboratory of Agricultural Genetically Modified Organisms Traceability,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部植物生态环境安全监督检测中心(武汉)/农业农村部农业转基因生物溯源重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2024年第1期84-91,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金面上项目(32171959)。

摘　　要：为验证单壁碳纳米管(SWNT)介导的新型的植物遗传转化方法的有效性,利用羧基化的单壁碳纳米管(COOH-SWNT)为原材料,构建了聚乙烯亚胺(PEI)修饰的羧基化单壁碳纳米管复合物(PEI-SWNT),探索验证PEI-SWNT复合物对外源基因的结合比例以及保护作用,使用PEI-SWNT复合物将RED以及UBQ10-EGFP等荧光质粒转化到油菜的原生质体和烟草叶片中。结果表明,PEI-SWNT纳米材料成功与外源目的基因结合,并对外源目的基因进行保护,使其免于核酸酶的降解,PEI-SWNT携带目的基因进入受体细胞后,可以成功地在受体细胞内进行基因表达。To verify the effectiveness of a plant genetic transformation method mediated by single-walled carbon nanotubes(SWNT),binding ratio and protection of PEI-SWNT(polyethyleneimine single-walled carbon nanotube complexes)to exogenous genes were investigated and veritied.Carboxylated single-walled carbon nanotubes(COOH-SWNT)were used as raw material to construct PEI-SWNT modified with polyethyleneimine(PEI).Then PEI-SWNT complex was used to transform fluorescent plasmids(eg.RED and UBQ10-EGFP)into oilseed rape protoplasts and tobacco leaves.Results showed that PEI-SWNT nanomaterials successfully bind to the exogenous target genes and protect them from nuclease degradation.PEI-SWNT was found to carry the target genes into recipient cells and could successfully express them in recipient cells.

关 键 词：油菜 烟草 遗传转化 单壁碳纳米管 聚乙烯亚胺单壁碳纳米管复合物(PEI-SWNT) 植物基因工程 

分 类 号：S572[农业科学—烟草工业]