油菜核盘菌(Sclerotinia sclerotiorum)对戊唑醇的抗药性风险评估  

Resistance risk for tebuconazole against Sclerotinia sclerotiorum

作　　者：赵丽丽 李子钦 史志丹 牟春雨 皇甫海燕 齐桂强 宋培玲 燕孟娇 皇甫九茹 杨永青 ZHAO Li-li;LI Zi-qin;SHI Zhi-dan;MU Chun-yu;HUANGFU Hai-yan;QI Gui-qiang;SONG Pei-ling;YAN Meng-jiao;HUANGFU Jiu-ru;YANG Yong-qing(Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,China;Tenihe Co.LTD,Hulun buir State Farm Group,Hulun buir 021024,China;College of Life Sciences of Inner Mongolia University,Hohhot 010021,China)

机构地区：[1]内蒙古自治区农牧业科学院植物保护研究所,内蒙古呼和浩特010031 [2]呼伦贝尔农垦集团特泥河农牧场有限公司,内蒙古呼伦贝尔021024 [3]内蒙古大学生命科学学院,内蒙古呼和浩特010021

出　　处：《中国油料作物学报》2024年第1期182-190,共9页Chinese Journal of Oil Crop Sciences

基　　金：内蒙古科技重大专项(2020ZD0005-0202);中国科学院战略性科技先导专项A(XDA26050101-01)。

摘　　要：为明确油菜核盘菌(Sclerotinia sclerotiorum)对杀菌剂戊唑醇的抗药性风险,通过生长速率法测定了119株核盘菌对戊唑醇的敏感性基线,通过紫外诱导结合药剂筛选获得2株抗药性菌株,开展了抗药性遗传稳定性、菌丝生长速率、产菌核能力、致病力、靶基因序列分析及表达量测定等研究。结果显示:119株油菜核盘菌对戊唑醇的EC50值范围为0.0121~0.8907μg/mL,平均值为0.2403±0.1365μg/mL,呈单峰曲线分布,未发现田间抗药性菌株;经室内诱导获得2株抗药性菌株19AH-5A、19AH-5C,对戊唑醇的EC50值分别是野生型敏感菌株的5.857和12.903倍;此外,抗药性菌株19AH-5C的生物学特性与敏感菌株无显著差异,而19AH-5A的致病力下降严重;2株抗药性菌株中戊唑醇靶标蛋白的编码基因CYP51的碱基序列与敏感菌株完全一致,未发现可引起氨基酸改变的碱基突变,但在戊唑醇培养条件下,抗药性菌株19AH-5C中靶基因CYP51的表达量显著上调3.72倍。油菜核盘菌在戊唑醇选择压力下可产生抗药性菌株,根据119株核盘菌的敏感性基线以及抗性菌株的环境适合度研究结果判断,油菜核盘菌对戊唑醇存在中等抗药性风险,因此使用戊唑醇防治油菜菌核病时应持续开展病原菌抗药性水平监测,同时可将不同作用类型杀菌剂交替使用以延缓田间抗药性的发生。In order to identify the risk of tebuconazole resistance of Sclerotinia sclerotiorum,susceptibility baseline of 119 strains of S.sclerotiorum to tebuconazole was established by growth rate method,and two resistant strains were obtained by UV induction combined with drug screening.Genetic stability of resistance,mycelial growth rate,sclerotinia production ability,pathogenicity,sequence analysis and expression measurement of target gene of resistant strains were studied.Results showed that EC50 values of all strains ranged from 0.0121 to 0.8907μg/mL,with an average of 0.2403±0.1365μg/mL,showing a unimodal curve distribution.No field resistant strains were detected.Two resistant strains 19AH-5A and 19AH-5C were obtained by indoor induction,and their EC50 values of tebuconazole were increased by 5.857 and 12.903 times,respectively.In addition,the biological characteristics of 19AH-5C strain were not significantly different from those of susceptive strain,while the pathogenicity of 19AH-5A was seriously degraded.The sequence of CYP51 gene encoding the target protein of tebuconazole in the two resistant strains were completely consistent with that in susceptive strain,and no base mutation resulting in amino acid change was found.However,the relative expression of CYP51 gene in the resistant strain 19AH-5C was significantly up-regulated by 3.72 times in condition treated with tebuconazole.Under the selective pressure of tebuconazole,it was suggested that S.sclerotiorum had a moderate resistance risk to tebuconazole.Therefore,when using tebuconazole to control S.sclerotiorum,resistance level of the pathogen is recommended to be monitored continuously,and fungicides of different action mode could be used alternately in rapeseed production to delay the occurrence of fungicide resistance in fields.

关 键 词：油菜 核盘菌 戊唑醇 抗药性 

分 类 号：S432.4[农业科学—植物病理学]