肉桂子化学成分鉴定、总黄酮提取工艺优化及其抗氧化活性研究  

Identification of Chemical Constituents,Optimization of Total Flavonoids Extraction Process,and Study on Antioxidant Activity of Fructus Cinnamomi cassiae immaturi

作　　者：黄梦 刘宏炳[1,2] 杨珍 燕雪花[1,2] HUANG Meng;LIU Hong-bing;YANG Zhen;YAN Xue-hua(College of traditional Chinese medicine,Xinjiang Medical University,Urumqi 830017,China;Key Laboratory of Famous Medical Formulas and Characteristic Prescriptions in Xinjiang,Xinjiang Medical University,Urumqi 830017,China)

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830017 [2]新疆医科大学新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830017

出　　处：《化学试剂》2024年第3期56-65,共10页Chemical Reagents

基　　金：新疆维吾尔自治区药品监督管理局科研项目(20210012)。

摘　　要：采用超高效液相色谱串联质谱法(UHPLC-MS/MS)鉴定肉桂子化学成分;基于单因素实验,采用响应面法优化肉桂子总黄酮提取工艺及含量测定;通过DPPH法、铁离子还原能力法评价其体外抗氧化活性。结果表明:肉桂子鉴定出135种化学成分;总黄酮最佳提取工艺为乙醇体积分数42%、料液比1∶36(g/mL)、功率350 W、超声时长43 min,在此条件下,总黄酮含量为377.90 mg/g;S4总黄酮含量最高,S18总黄酮含量最低;总黄酮对DPPH·和Fe^(3+)·均表现出较好的清除能力。综上,肉桂子含有黄酮类、萜类、酚类、生物碱等多种成分,该总黄酮提取工艺合理,不同批次肉桂子总黄酮均有一定的抗氧化作用。This study utilized ultrahigh performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)to identify the chemical components of Fructus Cinnamomi cassiae immaturi.Based on single factor experiments,response surface methodology was used to optimize the extraction process of total flavonoids from Fructus Cinnamomi cassiae immaturi.Based on this extraction process,the content of total flavonoids was determined.Its in vitro antioxidant activity was evaluated through DPPH method and iron ion reduction ability method.The results showed that 135 chemical components were identified from Fructus Cinnamomi cassiae immaturi.The optimal extraction conditions were as follows:ethanol volume fraction of 42%,solid-liquid ratio of 1∶36(g/mL),power of 350 W,and extraction time of 43 minutes.Under these conditions,the total flavonoid content is 377.90 mg/g.It was found that S4 has the highest total flavonoid content,while S18 has the lowest total flavonoid content,and total flavonoids showed good scavenging ability for both DPPH and Fe^(3+).In summary,the compounds of Fructus Cinnamomi cassiae immaturi consist of flavonoids,terpenoids,phenols,alkaloids,etc.The extraction process of total flavonoids is reasonably designed,and total flavonoids of different batches of Fructus Cinnamomi cassiae immaturi have certain antioxidant effects.

关 键 词：肉桂子 超高效液相色谱-串联质谱法 响应面 总黄酮 抗氧化 

分 类 号：TQ461[化学工程—制药化工]