温敏印迹柱对薯蓣皂素的选择保留和控制缓释  

Selective Retention and Controlled Release Behavior of a Temperature-Sensitive Imprinted Polymer Monolith for Diosgenin

作　　者：刘美君 彭雨婷 肖真仪 周小辉 简宇轩 毛琳 李辉[1,2] LIU Meijun;PENG Yuting;XIAO Zhenyi;ZHOU Xiaohui;JIAN Yuxuan;MAO Lin;LI Hui(College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China;National and Local United Engineering Laboratory of Integrative Utilization of Eucommia Ulmoides,Jishou University,Jishou 416000,Hunan China)

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]吉首大学国家与地方联合杜仲工程实验室,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2024年第1期48-55,共8页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(21865011);湖南省大学生省级创新课题(S202110531050);湘西自治州科技创新计划重点项目(2019SF2003);吉首大学本科生创新课题(JDXB2022024)。

摘　　要：以薯蓣皂素为模板,N-异丙基丙烯酰胺和α-甲基丙烯酸为混合功能单体制备1种温敏印迹聚合物,将其装填于不锈钢柱管中,在色谱模式下研究了温敏印迹柱对目标分子的保留和控制释放行为,同时探究了柱温、洗脱剂组成、pH值、离子强度等因素对分子印迹柱选择保留及控制释放的影响.结果表明,柱温对分子印迹材料的保留和控制释放具有显著影响.当色谱柱温为303 K时(临界温度附近),分子印迹柱对模板具有最强保留能力;当柱温高于或低于临界温度时,分子印迹柱的保留能力减弱,模板释放速率加快.洗脱液组成、pH值、离子强度也会影响印迹材料的保留和释放,在303 K柱温下,用0.001 mol/L缓冲液(pH值7.4)进行洗脱,释放时间高达1 050 min.A temperature-sensitive imprinted polymer was prepared with diosgenin as the template and withα-methacrylic acid(MAA)and N-Isopropylacrylamide(NIPAM)as mixed-functional monomer in present work.Then,this imprint polymer was filled into an empty stainless steel tube to produce a molecularly imprinted polymer column and the retention and controlled release behavior for this column toward the template as a drug molecule were evaluated in chromatographic model.The influence of column temperature,effluent composition and pH,and effluent ion strength on the retention selectivity and controlled release behavior for this imprint column was investigated.Results indicated that column temperature possessed significant influence on retention and controlled-release behavior for the imprint column.When it was set at 303 K(near critical temperature of this imprinted polymer),the column had the strongest retention capacity toward the template.Moreover,when column temperature was higher and/or lower than 303 K,a decreased retention capacity and an accelerated template release for the imprint column was observed.In addition,effluent composition,pH and effluent ion strength also affect the retention and controlled-release for this imprint column.At 303 K column temperature and with effluent of 0.001 mol/L(pH 7.4),the release time was as long as 1050 min.

关 键 词：分子印迹 温敏印迹柱 薯蓣皂素 保留行为 药物控制释放 

分 类 号：TQ460.1[化学工程—制药化工]