MDCK细胞无血清悬浮培养技术在流感疫苗研究与生产中的应用进展  

The Application Progress of Serum-free Suspension Culture Technology of MDCK Cells in Influenza Vaccine Study and Production

作　　者：靳莉武 张震宇 靳冬武 马花 马玉梅 乔自林[1,4,5] 王家敏[1,4,5] JIN Li-wu;ZHANG Zhen-yu;JIN Dong-wu;MA Hua;MA Yu-mei;QIAO Zi-lin;WANG Jia-min(Engineering Research Center for Key Technologies and Industrialization of Cell-Based Vaccines,Ministry of Education,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030;Lanzhou Lark Biotechnology Co.Ltd,Lanzhou 730010;College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030;Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030;Gansu Tech Innovation Center of Animal Cell,Biomedical Research Center,Northwest Minzu University,Lanzhou 730030)

机构地区：[1]西北民族大学生物医学研究中心,细胞基质疫苗关键技术与产业化教育部工程研究中心,兰州730030 [2]兰州百灵生物技术有限公司,兰州730010 [3]西北民族大学生命科学与工程学院,兰州730030 [4]西北民族大学生物医学研究中心,生物工程与技术国家民委重点实验室,兰州730030 [5]西北民族大学生物医学研究中心,甘肃省动物细胞技术创新中心,兰州730030

出　　处：《生物技术通报》2024年第2期38-47,共10页Biotechnology Bulletin

基　　金：甘肃省青年科技基金计划(22JR11RA239);西北民族大学中央高校基本科研业务费项目(31920230001)。

摘　　要：流感是一种常见的呼吸道疾病,由流感病毒引起,接种疫苗是预防流感的有效手段。MDCK细胞(Madin-Darby Canine Kidney Cells)具有易于培养和高产量的特点,可以支持流感病毒的复制和增殖,被广泛用于流感疫苗的研究和生产。随着对流感病毒研究的不断深入,为了进一步拓展MDCK细胞用于流感病毒研究和工业应用的能力,研究人员开始发展MDCK细胞无血清全悬浮培养技术。通过长期的培养和优化,驯化的MDCK悬浮细胞株可以更好地适应流感病毒的生长环境,提高流感病毒的产量和感染性。总之,MDCK细胞无血清悬浮培养技术在流感病毒研究和工业应用中发挥着重要作用,为流感疫苗生产和抗流感药物研发提供更好的工具。同时也必须重视MDCK细胞的安全性,采取合适的措施来确保其应用的安全性和可靠性。Influenza is a common respiratory disease caused by influenza viruses.MDCK(Madin-Darby Canine Kidney)cells have the characteristics of easy culture and high production,which can support the replication and proliferation of influenza viruses,and are widely used in the research and production of influenza viruses.With the deepening of research on influenza virus,and in order to further optimize the ability of MDCK cells to be used for influenza virus research and industrial application,researchers began to develop the domestication technology of MDCK cells.Through long-term cultivation and selection,the domesticated MDCK cells strain can better adapt to the growth environment of influenza virus,and improve the production and infectivity of influenza virus.In conclusion,the domestication technology of MDCK cells plays an important role in influenza virus research and industrial application,which may improve the production and infectivity of influenza viruses and provide better tools for influenza vaccine production and anti influenza drug development.Concurrently we have to also pay attention to the safety of MDCK cells and take appropriate measures to ensure the safety and reliability of its application.

关 键 词：MDCK细胞 细胞悬浮驯化 无血清培养基 流感疫苗 安全性 

分 类 号：Q813.11[生物学—生物工程] R392-33[医药卫生—免疫学]