黄龙病发病芦柑根际土壤细菌群落组成与多样性特征  

Bacterial Diversity and Structure in Rhizosphere Soil of Citrus Infested with Huanglongbing

作　　者：雷美玲 饶文华 胡进锋[2] 岳琪 吴祖建[1] 范国成[2] LEI Mei-ling;RAO Wen-hua;HU Jin-feng;YUE Qi;WU Zu-jian;FAN Guo-cheng(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002;Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013)

机构地区：[1]福建农林大学植物保护学院,福州350002 [2]福建省农业科学院植物保护研究所,福州350013

出　　处：《生物技术通报》2024年第2期266-276,共11页Biotechnology Bulletin

基　　金：国家重点研发计划(2021YFD1400800);福建省农业科学院项目(XTCXGC2021011,CXTD2021002-1)。

摘　　要：【目的】本研究旨在研究福建南平地区芦柑(Citrus reticulata)植株根际土壤细菌群落,并分析黄龙病对其细菌群落的影响,为南平地区芦柑黄龙病的防控提供科学依据。【方法】采用五点法采集了健康和黄龙病发病的芦柑植株根际土壤样本,通过扩增子测序技术和生物信息学方法,深入研究了黄龙病发病芦柑根际土壤细菌群落的多样性和组成变化,分析了土壤理化因子与细菌群落的关联性。【结果】研究结果表明,黄龙病发病的芦柑植株根际土壤细菌群落的多样性略高于健康植株。黄龙病发病芦柑根际土壤中的变形菌门相对丰度明显升高,而放线菌门相对丰度显著降低。在属水平上,与健康植株相比,黄龙病发病芦柑植株根际土壤细菌群落中蔷薇属和嗜酸性杆菌属的相对丰度显著增加,与速效钾、有效磷以及有机质呈现显著负相关,而与pH值呈现显著正相关。相反地,康奈斯氏杆菌属和褚氏杆菌属的相对丰度明显低于健康植株,并与速效钾、有效磷以及有机质呈现显著正相关,同时与p H值呈现显著负相关。【结论】黄龙病菌感染改变了柑橘土壤理化特性,并显著减少根际土壤中有益细菌的相对丰度,从而导致了芦柑植株根际细菌群落的多样性和组成出现显著变化。与此同时,黄龙病发病的芦柑植株可能通过招募固氮和促进植物根际生长的有益细菌来对抗病原菌的侵害。【Objective】This study aims to investigate the rhizosphere soil bacterial community of Citrus reticulata plants in the Nanping region of Fujian province,China.The objective is to analyze the impact of Huanglongbing(HLB)disease on the bacterial community and provide a scientific basis for HLB management strategies in the Nanping region.【Method】We collected root-zone soil samples from both healthy and HLB-affected C.reticulata plants using the five-point sampling method.We used amplicon sequencing technology and bioinformatics methods to investigate the changes in diversity and composition of the bacterial community in the root-zone soil of HLB-affected C.reticulata plants.Additionally,we analyzed the correlation between soil physicochemical factors and the bacterial community.【Result】The findings of the study demonstrate that the diversity of the bacterial community in the root-zone soil of C.reticulata plants affected by HLB was slightly higher when compared to the microbiota of healthy plants.Specifically,there was a significant increase in the relative abundance of the Proteobacteria,and a significant decrease in the relative abundance of the Actinobacteriota in the root-zone soil of HLB-affected C.reticulata plants.At the genus level,it was observed that the relative abundance of the Roseiarcus and Acidiphilium significantly increased in the bacterial community of HLB-affected C.reticulata plants.Furthermore,these genera presented a significant negative correlation with available potassium,available phosphorus,and organic matter,while showing a significantly positive correlation with pH values.Conversely,the relative abundance of the Conexibacter and Chujaibacter were significantly lower in HLB-affected plants than healthy ones.Additionally,these genera demonstrated a significantly positive correlation with available potassium,available phosphorus,and organic matter,but a significant negative correlation with pH values.【Conclusion】The infection of the HLB pathogen altered the physicochemical pro

关 键 词：黄龙病 柑橘 高通量测序 根际土壤 细菌群落 差异物种 环境因子 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治] S154.3[农业科学—植物保护]