木霉菌与多种杀菌剂生物相容性研究  

A study on biocompatibility of Trichoderma spp.with various fungicides

作　　者：李歆建 杨成 刘晓昂 陈芝波 宋泽军 莫泽刚 陈雨轩 王杰[2] 罗倩茜 LI Xinjian;YANG Cheng;LIU Xiaoang;CHEN Zhibo;SONG Zejun;MO Zegang;Chen Yuxuan;Wang Jie;LUO Qianqian(Qiannan Branch of Guizhou Tobacco Company,Duyun,Guizhou 558000,China;Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266000,China)

机构地区：[1]贵州省烟草公司黔南州公司,贵州都匀558000 [2]中国农业科学院烟草研究所,山东青岛266000

出　　处：《生物灾害科学》2024年第1期67-72,共6页Biological Disaster Science

基　　金：贵州省烟草公司科技项目(2023522700240236)。

摘　　要：【目的】探究哈茨木霉(Tcomp、TH1和TH3)和绿色木霉(TV1)菌株与防治种子传播和叶部病害的不同活性成分杀菌剂之间的生物相容性,以期在植保工作中减少化学农药的使用,响应综合防治的植保方针。【方法】通过菌丝生长、分生孢子产量、孢子萌发率评估7种不同杀菌剂(包含复配)对4株木霉菌株的抑制作用强弱,以期筛选出与之生物相容性最好的农药。【结果】从菌丝生长方面来看,4种木霉菌株与福满双的相容性最差,在中、高浓度下相容性均为0,4种木霉菌株与环酰菌胺的相容性最好,从14 d后,相容性均为100%。从孢子产量和萌发方面来看,4种木霉菌株与环酰菌胺的相容性最好,在最高浓度下,最低的相容性达到了22.5%。4种木霉菌株与嘧霉胺的相容性最差,各种浓度下4种木霉菌株的相容性均不超过20%,最低的为0。【结论】环酰菌胺与4株木霉菌株的相容性最好,其次为嘧菌胺。百菌清、吡唑醚菌酯+啶酰菌胺、嘧菌环胺+咯菌腈与4株木霉菌株的相容性相当,均为中等偏高,而福满双和嘧霉胺与4株木霉菌株的相容性最差。[Objective]This study was conducted to determine the biocompatibility of Trichoderma harziensis(Tcomp,TH1 and TH3)and Trichoderma viridis(TV1)strains with different active ingredient fungicides for control of seed spread and leaf diseases in vitro,so as to reduce the use of chemical pesticides in plant protection and respond to the plant protection policy of integrated control.[Method]The effects of 7 different pesticides on 4 Trichoderma strains were evaluated by the mycelium growth,conidial production and conidial germination rate.[Result]The results showed that from the perspective of mycelial growth,the compatibility of four Trichoderma strains with thiram was the worst,0 at medium and high concentrations,and that with fenhexamid was the best,100%after 14 days.In terms of spore yield and germination,the compatibility of four Trichoderma strains with fenhexamid was the best,the lowest compatibility having reached 22.5%at the highest concentration;which with pyrimethanil was the worst,under various concentrations being less than 20%and the lowest being 0.[Conclusion]The compatibility of 4 Trichoderma strains with fenhexamid was the best,followed by that with mepanipyrim.The compatibility of that with chlorothalonil,pyraclostrobin+boscalid,cyprodinil+fludioxonil was moderate and high,while,that with thiram and pryimethanil was the worst.

关 键 词：哈茨木霉 绿色木霉 杀菌剂 生物相容性 

分 类 号：S482.2[农业科学—农药学]