一株高效石油降解菌的筛选及降解能力初探  

Screening and Degradation Capability of a Highly Efficient Petroleum-Degrading Bacterium

作　　者：王嘉彬 蔡昌福 秦达 李雪菲 张春龙 余丽芸[1] Wang Jiabin;Cai Changfu;Qin Da;Li Xuefei;Zhang Chunlong;Yu Liyun(Key Laboratory of Environmental Microbiology and Recycling in Cold Regions of Heilongjiang Province,College of Life Science and Technology,Heilongjiang Bayi Agricultural Reclamation University,Daqing 163000,Heilongjiang,China;Daqing Yilai Inspection and Testing Technology Service Co.,Ltd.,Daqing 163000,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江省寒区环境微生物及循环利用重点实验室,黑龙江大庆163000 [2]大庆亿莱检验检测技术服务有限公司,黑龙江大庆163000

出　　处：《绿色科技》2024年第2期209-215,共7页Journal of Green Science and Technology

摘　　要：为了有效降解石油开采过程中产生的含油污泥中的石油,达到油泥资源再利用的目的,选育高效石油降解菌,为石油污染生物修复提供菌种资源和技术支持。通过连续富集传代培养,从页岩油泥中分离出一株高效石油降解菌YH1-1,经过形态学、生理生化以及16S rRNA序列分析,鉴定YH1-1为醋酸钙不动杆菌(Acinetobacter calcoaceticus)。该菌在温度30℃、初始培养pH值7.0条件下降解质量浓度50 g/L的油泥,经气相色谱(GC)分析,28 d降解率可达87%,说明该菌可有效降解石油烃。因此,醋酸钙不动杆菌YH1-1在开发研制石油污染生物修复菌剂方面有较好的应用前景。In order to effectively degrade the oil in the oil sludge produced by oil exploitation and to achieve the purpose of recycling the oily-sludge resources,the high-efficiency degrading petroleum bacteria in the oil sludge were isolated and identified,which provided the strain resources and technical support for the biological remediation of oil pollution.The strain YH1-1 was isolated from Shale oily sludge by enrichment of the continuously passage culture.The YH1-1 was identified as Acinetobacter calcoaceticus by morphology,physiology and biochemistry,and 16S rRNA sequence analysis.At days 28,the degradation rate of the bacterium YH1-1 reached 87% by gas chromatography(GC) detection under the conditions of 30℃,an initial pH 7.0,and a mass concentration of oil sludge of 50g/L.The results demonstrated that the bacteria could effectively degrade petroleum hydrocarbons.Therefore,Acinetobacter calcoaceticus YH1-1 has a good application prospect in the development of petroleum pollution bioremediation agents.

关 键 词：石油降解菌 分离鉴定 生物降解 醋酸钙不动杆菌 

分 类 号：X53[环境科学与工程—环境工程]