贝绒刺蛾Phocodermabetis线粒体全基因组测序与分析  

Sequencing and analysis of the complete mitochondrial genome of Phocodermabetis(Lepidoptera:limacodidae)

作　　者：俞旭东 李朝品[1,2] YU Xudong;LI Chaopin(Department of Parasitology,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院医学寄生虫学教研室,安徽芜湖241002 [2]芜湖市螨类检验与防控工程研究中心,安徽芜湖241002

出　　处：《皖南医学院学报》2024年第1期90-95,共6页Journal of Wannan Medical College

基　　金：国家级大学生创新创业训练计划项目(202110368033)。

摘　　要：目的:测定贝绒刺蛾Phocodermabetis线粒体基因组全序列,并分析其线粒体基因组碱基组成、密码子使用偏好性等特征,探讨刺蛾科在鳞翅目(Lepidoptera)中的系统发育地位。方法:利用Illumina高通量测序技术测得贝绒刺蛾完整线粒体基因组序列。通过Mitos2、Geneious v8.0.4和Phylosuite1.2.2等生物信息软件对线粒体基因组进行注释分析,并基于26种鳞翅目昆虫的13个蛋白质编码基因,使用最大似然法和贝叶斯法构建系统发育树。结果:贝绒刺蛾线粒体全基因组序列长14919 bp(AT:80.88%),包括13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个AT富集区。刺蛾科线粒体基因组AT含量、AT-skew及GC-skew均表现出极高的相似性。系统发育树表明,卷夜蛾科(Tortricidae)+[透翅蛾科(Sesiidae)+木蠹蛾科(Cossidae)]+[刺蛾科(Limacodidae)+斑蛾科(Zygaenidae)]。结论:刺蛾科(Limacodidae)与斑蛾科(Zygaenidae)互为姊妹群,同属于斑蛾总科Zygaenoidea。贝绒刺蛾属于刺蛾科并与绿刺蛾属(Parasa)亲缘关系较为接近。Objective:To determine the complete sequences of the mitogenome of Phocodermabetis,and analyze the characteristics of the base composition and codon usage bias of mitogenome,so as to investigate the phylogenetic position of Phocodermabetis in Lepidoptera.Methods:In this study,the complete mitochondrial sequence of Phocodermabetis was sequenced by Illumina high-throughput sequencing technique.Then the mitochondrial sequence were annotated and analyzed using bioinformatics software,including Mitos2,Geneious v8.0.4,and Phylosuite 1.2.2.Finally,two phylogenetic trees were constructed by both maximum likelihoodandBayesianmethods based on 13 protein coding genes of 26 species of Lepidoptera.Results:The complete mitochondrial sequence of Phocodermabetis was 14919bp(AT:80.88%).The mitogenome consisted of 13 protein coding genes,22 tRNA genes,2 rRNA genes,and an AT enrichment region.The AT content,AT-skew,and GC-skew of the mitogenome of Phocodermabetis showed higher similarity.The phylogenetic trees revealed[Tortricidae+(Sesiidae+Cossidae)+(Limacodidae+Zygaenidae)].Conclusion:The family Limacodidae and the family Zygaenidae represent sister group,belonging to the superfamily Zygaenoidea,and Phocodermabetis is in close genetic relationship with the genus Parasa.

关 键 词：贝绒刺蛾 高通量测序 刺蛾科 线粒体基因组 系统发育 

分 类 号：R384[医药卫生—医学寄生虫学] Q969.431.2[医药卫生—基础医学]