Serratia sp.对铜绿微囊藻、斜生栅藻的溶藻效果  

Algae-lysing Effect of Algicidal Bacterium Serratia sp.on Microcystis aeruginosa and Scenedesmus obliquus

作　　者：洪桂云 汪涛 朱慧 马少雄 HONG Guiyun;WANG Tao;ZHU Hui;MA Shaoxiong(Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province/College of Environment and Energy Engineering,Anhui Jianzhu University,Hefei 230601,China)

机构地区：[1]安徽建筑大学环境与能源工程学院/水污染控制与废水资源化安徽省重点实验室,安徽合肥230601

出　　处：《广东海洋大学学报》2024年第1期76-83,共8页Journal of Guangdong Ocean University

基　　金：安徽省自然科学基金(No.1908085141ME);安徽建筑大学校级教研项目(No.2021jy27)。

摘　　要：【目的】探讨一株溶藻菌S6(Serratia sp.)对富营养化水中优势蓝藻和绿藻的溶藻效果,为有害藻类的生物控制提供参考。【方法】从富营养化水体中分离到一株溶藻菌S6(Serratia sp.,GenBank登录号为KY462187),将不同浓度菌液分别加至铜绿微囊藻(Microcystis aeruginosa)、斜生栅藻(Scenedesmus obliquus)以及两者以浓度比1∶1混合的藻液(下称“混合藻”)中,进行溶藻实验,测定14 d内实验体系中藻的生物量、叶绿素a、氨氮和总磷(TP)浓度,分析溶藻菌S6对不同藻类的溶藻效果以及对水体中氮磷的影响;运用光谱技术分析溶藻产物特性。【结果与结论】溶藻菌S6对铜绿微囊藻、斜生栅藻和混合藻的溶藻效果较佳,且菌液浓度越高,溶藻效果越佳,其中3组原菌液(2×10^(9)mL^(-1))处理组14 d内的溶藻率分别为89.4%、80%、78.6%。溶藻菌S6对3种实验体系氨氮和TP浓度影响较大,原菌液处理组变化最明显,其中铜绿微囊藻藻液氨氮和TP质量浓度分别下降21.24、6.21 mg/L;斜生栅藻藻液氨氮和TP质量浓度分别下降13.19、7.54 mg/L;混合藻液中氨氮和TP质量浓度分别下降10.81、11.85 mg/L。傅里叶红外光谱、紫外光谱和三维荧光光谱分析表明,铜绿微囊藻的溶藻产物中主要含类色氨酸、类富里酸和类腐殖酸物质,斜生栅藻的溶藻产物中主要含类色氨酸和类腐殖酸物质。【Objective】The aim of this paper is to investigate the algae-lysing effect of an algaolytic bacterium S6(Serratia sp.)on dominant cyanobacteria and green algae in eutrophic water.【Method】The algaolytic bacterium S6(Serratia sp.,accession No.KY462187)was isolated from the eutrophic water;the bacterium at different concentrations was added to the algal solutions of Microcystis aeruginosa,Scenedesmus obliquus,and a mixture of the two in the ratios of 1∶1(Hereinafter referred to as“mixed algae”).The algal biomass,concentration of chlorophyll a,NH_(3)-N and TP of the experimental systems were measured,and the algae-lysing effect on different algae and effect on nitrogen and phosphorus of the experimental systems were analyzed in a fortnight.Then,algae-lysing products were analyzed by spectral technology of these experimental systems.【Result and Conclusion】The strain S6 had a desirable algae-lysing effect on M.aeruginosa and S.obliquus under monoculture and coculture,the higher the concentrations of bacterium,the better the algae-lysing effect,dissolving M.aeruginosa,S.obliquus and the mixed algae under coculture with a removal rate of 89.4%,80%and 78.6%in the three original bacterial suspensions.Furthermore,the concentrations of NH_(3)-N and total phosphorus(TP)of the three experimental systems existed fluctuations significantly,those of the M.aeruginosa decreased by 21.24 and 6.21 mg/L,those of the S.obliquus decreased by 13.19 and 7.54 mg/L,those of the mixed algae decreased by 10.81 and 11.85 mg/L.The algae-lysing products of the three experimental systems were analyzed by infrared spectroscopy,ultraviolet spectroscopy and three dimensional fluorescence spectroscopy scanning,and it was found that the algae-lysing products of M.aeruginosa mainly contained tryptophan-like,fulvic acid-like and humic acid-like substances,while the algae-lysing products of S.obliquus mainly contained tryptophan-like and humic acid-like substances.

关 键 词：铜绿微囊藻 斜生栅藻 溶藻菌 Serratia sp. 溶藻产物 光谱分析技术 

分 类 号：X52[环境科学与工程—环境工程]