儿童脓毒症血浆代谢物分析  

Analysis of plasma metabolites in sepsis in children

作　　者：欧俊斌[1] 林敏欣 李小琳[1] 刘玉玲[1] 梁巧 OU Junbin;LIN Minxin;LI Xiaolin;LIU Yuling;LIANG Qiao(Zhongshan Boai Hospital,Zhongshan 528403,China)

机构地区：[1]中山市博爱医院儿科,广东中山528403 [2]中山市博爱医院新生儿科,广东中山528403

出　　处：《中国中西医结合儿科学》2024年第1期42-46,共5页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：中山市医学科研项目(2020J153)。

摘　　要：目的 分析脓毒症患儿血浆代谢物的水平,寻找儿童脓毒症血浆差异代谢物。方法 选取2019年10月至2021年11月中山市博爱医院收治的37例脓毒症早期患儿为观察组,在入院24 h内采血,选择同期38例健康儿童为对照组,检测两组血浆中11项氨基酸及31项酰基肉碱,比较两组血浆代谢物水平的差异。结果 通过主成分分析和正交偏最小二乘法-判别分析(OPLS-DA)显示两组血浆氨基酸及酰基肉碱代谢谱差异显著,氨基酸分析中,苯丙氨酸、缬氨酸、亮氨酸、甘氨酸明显增高,酰基肉碱分析中,丁酰肉碱、异戊酰肉碱明显增高,游离肉碱、肉豆蔻酰肉碱、棕榈酰肉碱、棕榈烯酰肉碱、十八碳酰肉碱、十八碳烯酰肉碱及十八碳二烯酰肉碱明显减少。结论 脓毒症患儿的差异代谢物提示机体存在代谢紊乱,并有线粒体功能障碍,可作为儿童脓毒症的生物标志物。Objective To find the differential metabolites in children with sepsis by analyzing the levels of serum metabolites.Methods Serum samples were collected in 24h after admission from 37 children with early sepsis as the observation group,who were hospitalized in Zhongshan Boai Hospital from October 2019 to November 2021,and 38 healthy children were enrolled as the control group during the same period.The levels of 11 kinds of amino acids and 31 kinds of acylcarnitine in plasma were detected and the differences in serum metabolites were compared between two groups.Results Principal component analysis(PCA) and orthogonal partial least squares diseriminant analysis(OPLS-DA) showed that there was significantly different metabolic profiling of plasma amino acids and acylcarnitine between observation group and control group;in the analysis of amino acid,the levels of phenylalanine,valine,leucine and glycine were significantly increased;in the analysis of acylcarnitine,the levels of butyrylcarnitine and isovalerylcarnitine were significantly increased,while the levels of l-carnitine,myristoylcarnitine,palmitoylcarnitine,hexadecenoyl carnitine,octadecanoylcarnitine,octadecaenoylcarnitine and octadecadienoylcarnitine were significantly decreased.Conclusion The differences in serum metabolites in children with sepsis is mainly associated with metabolic disorders,and there may be mitochondrial dysfunction,which can be used as biomarkers of sepsis in children.

关 键 词：脓毒症 代谢组学 儿童 

分 类 号：R725.5[医药卫生—儿科]