载铜离子自组装多肽抗菌眼用水凝胶的体外研究  

Study on antibacterial ophthalmice hydrogel loaded with copper ion self-assembled peptide in vitro

作　　者：张芯源 黄亚楠 代艳 张佳丽 王毅[1] 吕磊 ZHANG Xinyuan;HUANG Yanan;DAI Yan;ZHANG Jiali;WANG Yi;LÜLei(Sichuan Natural Medicines Mimetic Synthesis Engineering Research Centre,School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China)

机构地区：[1]西南交通大学生命科学与工程学院、四川省天然药物仿生合成工程研究中心,四川成都610031

出　　处：《华西药学杂志》2024年第1期13-17,共5页West China Journal of Pharmaceutical Sciences

摘　　要：目的制备与铜离子配位结合的自组装多肽抗菌眼用水凝胶。方法(RADA)_(4)自组装多肽与CuCl_(2)水溶液迅速混合均匀,形成稳定的(RADA)_(4)@Cu^(2+)水凝胶。对水凝胶进行SEM、EDS、FTIR及UV-Vi光度性能的表征。探究水凝胶对金黄色葡萄球菌和大肠杆菌的抗菌能力及对人角膜上皮细胞的细胞相容性。结果(RADA)_(4)@Cu_(2+)水凝胶具有良好的抗菌性和生物相容性。结论为更深层次的眼用凝胶应用研究打下了基础。OBJECTIVE To prepare a self-assembled peptide antimicrobial ophthalmic hydrogel ligated to copper ions.METHODS(RADA)_(4)self-assembled peptide was rapidly mixed with CuCl_2 aqueous solution to form a stable(RADA)_(4)@Cu^(2+)hydrogel.The hydrogels were characterised by SEM,EDS,FTIR and UV-Vi photometric properties.The antibacterial properties of hydrogel were investigated against Staphylococcus aureus and Escherichia coli,as well as the biocompatibility of the hydrogel with human corneal epithelial cells.RESULTS(RADA)_(4)@Cu^(2+)hydrogel had good antibacterial properties and biocompatibility.CONCLUSION It lays the foundation for the application study of ophthalmic gels.

关 键 词：(RADA)_(4) 自组装多肽 金属配位 水凝胶 角膜上皮细胞 抗菌 铜离子 生物相容性 

分 类 号：R94[医药卫生—药剂学]