三七总皂苷对调节性T细胞再分化为辅助性T细胞的影响  

Effects of total saponins of Panax notoginseng on re-differentiation of Treg into Th17 cells

作　　者：田锋祥 张明菲 申美玉 狄昱希 周玲玲[1] TIAN Fengxiang;ZHANG Mingfei;SHEN Meiyu;DI Yuxi;ZHOU Lingling(School of Pharmacy,Nanjing University of Traditional Chinese Medicine,Nanjing,Jiangsu,210023 P.R.China)

机构地区：[1]南京中医药大学药学院,江苏南京210023

出　　处：《华西药学杂志》2024年第1期33-36,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:82174306)。

摘　　要：目的考察三七总皂苷(PNS)调控调节性T细胞(Treg)再分化为辅助性T细胞17(Th17)的作用。方法分离C57/BL6小鼠脾细胞,并磁珠分选出CD4^(+)T细胞,进行体外培养,采用CCK-8法筛选PNS合适的给药浓度。建立Treg再分化为Th17的细胞模型,采用流式细胞术检测PNS对Treg再分化体系中Th17细胞分化比例的影响,酶联免疫吸附法检测体系上清中Th17特征性细胞因子白细胞介素(IL)-17A的水平,实时荧光定量聚合酶链式反应检测Th17特征性转录因子RORγt mRNA及炎症因子IL-17 mRNA的表达。结果PNS可显著降低Treg再分化体系中CD4^(+)IL-17^(+)Th17细胞的比例和IL-17A的分泌水平,抑制ROR-γt mRNA和IL-17 mRNA的表达。结论一定浓度的PNS可抑制Treg再分化为Th17,调控Treg/Th17平衡,进而防治类风湿关节炎等炎症免疫性疾病的发生和发展。OBJECTIVE To investigate the effect of total saponins of Panax notoginseng(PNS)on the trans-differentiation of Treg into Th17.METHODS The spleen cells of C57/BL6 mice were isolated and CD4^(+)T cells were cultured in vitro by magnetic beads sorting.The appropriate concentration of PNS was screened by CCK-8 method,based on the index of cell viability.The cell model of Treg trans-differentiation into Th17 was established.The effect of PNS on the differentiation ratio of Treg/Th17 cells was detected by using flow cytometry,the level of Th17 cell characteristic cytokine IL-17A was measured by ELISA,and the Th17 cell characteristic transcription factor RORγt mRNA and inflammatory factor IL-17 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction.RESULTS PNS could significantly inhibit the proportion of CD4^(+)IL-17^(+)Th17cells,the secretion of cytokine IL-17A and RORγt mRNA expression.CONCLUSION Certain concentrations of PNS can inhibit the trans-differentiation of Treg into Th17 and regulate the proportion balance of Treg/Th17 cells,and then prevent the occurrence and development of inflammatory immune diseases including rheumatoid arthritis.

关 键 词：类风湿关节炎 滑膜炎症 三七总皂苷 调节性T细胞 辅助性T细胞17 再分化 RORΓT 白细胞介素-17A 

分 类 号：R96[医药卫生—药理学]