植物药复方MYD-20抗金黄色葡萄球菌的作用及机制研究  

Study on the effects and mechanism of botanical compound MYD-20 against Staphylococcus aureus

作　　者：戴晓婷 陈思 周代红 茆灿泉[1] DAI Xiaoting;CHEN Si;ZHOU Daihong;MAO Canquan(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan,610031 P.R.China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《华西药学杂志》2024年第1期43-47,共5页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技厅科技计划重点项目(2022YFS0437)。

摘　　要：目的研究植物药复方MYD-20及其软膏剂对金黄色葡萄球菌的抑制作用及初步作用机制。方法采用肉汤稀释法测定复方MYD-20对金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林金黄色葡萄球菌Methicillin-resistant S.aureus(MRSA)临床菌株的最低抑菌浓度(MIC)、最低杀菌浓度(MBC);通过测定药物处理后菌液的碱性磷酸酶(AKP)活性、胞外大分子浓度变化以及扫描电子显微镜的观察结果确定复方MYD-20的抑菌作用机制;建立小鼠烫伤感染模型,探究MYD-20软膏剂的药效作用。结果MYD-20对金黄色葡萄球菌和MRSA表现出强抑菌活性,对金黄色葡萄球菌、MRSA的MIC和MBC均分别为1.0%、2.0%。经MYD-20处理后,菌液中AKP的活性、胞外大分子的含量均显著上升,结合扫描电子显微镜的观察结果,发现细菌的表面结构受到破坏。MYD-20软膏剂对皮肤深Ⅱ度烫伤感染的模型小鼠显示出显著的创口愈合作用;并降低了创口处炎症因子的表达。结论复方MYD-20对金黄色葡萄球菌和MRSA具有较强的抗菌作用,其作用机制与破坏细菌细胞膜、细胞壁的通透性和完整性,导致细胞内容物泄露有关。此外,MYD-20软膏剂能有效降低烫伤感染小鼠创口处的炎症水平,促进创口愈合。OBJECTIVE To investigate the inhibitory effect and preliminary mechanism of the botanical compound MYD-20 and its ointment preparation on Staphylococcus aureus.METHODS The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the clinical strains of Staphylococcus aureus and Methicillin-resistant S.aureus(MRSA)were determined by broth dilution method.The mechanism of antibacterial effect of MYD-20 was determined by the alkaline phosphatase activity and extracellular macromolecule concentration of the drug-treated bacterial solution as well as scanning electron microscopy.RESULTS MYD-20 showed strong antibacterial activity against S.aureus and MRSA,with MIC and MBC of 1.0%and 2.0%against both S.aureus and MRSA,respectively.AKP activity and extracellular macromolecule content in the bacterial solution were significantly increased after MYD-20 treatment,and combined with SEM observation,it could be found that the surface MYD-20 ointment showed significant wound healing effect and reduced the levels of inflammatory factors at the wounds in the model mice with deepⅡdegree skin scald infection.CONCLUSION The compound MYD-20 has a strong antibacterial effect on S.aureus and MRSA,and its mechanism is related to disrupting the permeability and integrity of bacterial cell membranes and cell walls,leading to leakage of cell contents.In addition,MYD-20 ointment can effectively reduce the level of inflammation at the wounds of scald-infected mice,as well as promote the healing effect of the wounds.

关 键 词：植物药复方 金黄色葡萄球菌 耐甲氧西林金黄色葡萄球 抑菌活性 梯度稀释法 软膏剂 最低抑菌浓度 最低杀菌浓度 深Ⅱ度烫伤感染 

分 类 号：R96[医药卫生—药理学]