中药炮制辅料炼蜜制备工艺及质量标准研究  

Preparation Process and Quality Standards of Honey Refining for Traditional Chinese Medicine Processing Excipients

作　　者：刘小鸣[1] 曹文文 王瑞生[2,3] 张振凌 LIU Xiaoming;CAO Wenwen;WANG Ruisheng;ZHANG Zhenling(The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou Henan China 450008;Henan University of Chinese Medicine,Zhengzhou Henan China 450046;Traditional Chinese Medicine Specialty Processing Technology Engineering Research Center of Henan Province,Zhengzhou Henan China 450008)

机构地区：[1]河南中医药大学第三附属医院,河南郑州450008 [2]河南中医药大学,河南郑州450046 [3]河南省中药特色炮制技术工程研究中心,河南郑州450008

出　　处：《中医学报》2024年第3期631-637,共7页Acta Chinese Medicine

基　　金：河南省药品监督管理局科技计划项目(2020DB125);河南中医药大学博士基金项目(RSBSJJ2019-09)。

摘　　要：目的:优化中药炮制辅料炼蜜的制备工艺,并建立炼蜜质量标准。方法:将加热温度、加热时间、加水量作为考察因素,采用L_(9)(3^(4))设计正交试验。根据水分含量、总糖含量,优选炼蜜最佳制备工艺。以最佳工艺制备炼蜜样品,鉴别其性状,检测其酸度、水分含量、灰分含量、相对密度等。采用高效液相色谱法检测炼蜜样品的果糖和D-葡萄糖含量。结果:炼蜜最佳制备工艺为取滤过的蜂蜜,加入5%蜂蜜质量的蒸馏水,加热温度为90℃,加热10 min。采用最佳工艺制备的炼蜜呈半透明状,浅黄色,无结晶,气芳香,味极甜。炼蜜中无淀粉或糊精掺入,且无发酵变酸现象。炼蜜水分含量为16.2176%~17.8195%,灰分含量为0.0523%~0.0870%,相对密度为1.4636~1.4660。炼蜜样品中果糖和D-葡萄糖含量总和为74.1879%~75.5785%,平均值为74.9453%;果糖含量/D-葡萄糖含量比值为1.26~1.31,平均比值为1.29。结论:本研究挖掘炼蜜的最佳制备工艺,该工艺稳定可靠,同时为炼蜜质量标准的制订提供依据。Objective:To optimize the preparation process of refining honey for traditional Chinese medicine processing excipients and establish quality standards.Method:An L_(9)(3^(4))orthogonal experiment was designed,with heating temperature,heating time and amount of water added considered as factors to investigate.Select the optimal preparation process for honey refining based on the moisture content and total sugar content.Prepare refined honey samples by using the best process to identify their characteristics as well as test their acidity,moisture content,ash content and relative density,etc.Detect the content of fructose and D-glucose in refined honey samples with high-performance liquid chromatography.Results:The optimal preparation process for honey refining is to take filtered honey,add 5%distilled water of honey quality,heat at 90℃for 10 minutes.The refined honey prepared by the best process is semi-transparent,light yellow,non-crystalline,fragrant,and extremely sweet in taste.There is no starch or dextrin added in the honey refining process,and there is no fermentation or acidification phenomenon.The moisture content of refined honey is 16.2176%-17.8195%,the ash content is 0.0523%-0.0870%,and the relative density is 1.4636-1.4660.The total content of fructose and D-glucose in refined honey samples ranges from 74.1879%to 75.5785%,with an average content of 74.9453%;The ratio of fructose content to D-glucose content is 1.26-1.31,with an average ratio of 1.29.Conclusion:This study explores the optimal preparation process for honey refining,which is stable and reliable to provide a basis for the formulation of honey refining quality standards.

关 键 词：炼蜜 中药炮制 制备工艺 质量标准 HPLC 炮制辅料 

分 类 号：R283.2[医药卫生—中药学]