抗菌肽对实验兔角膜损伤的疗效试验研究  

Study in Efficacy of Antimicrobial Peptides on Corneal Injury in Experimental Rabbits

作　　者：林仁钊 丁成城 陈瑜 李晋飞 陈嘉欣 苏雅琳 林飞 石达友[1] LIN Renzhao;DING Chengcheng;CHEN Yu;LI Jinfei;CHEN Jiaxin;SU Yalin;LIN Fei;SHI Dayou(College of Veterinary Medicine,South China Agricultural University,Guangzhou Guangdong 510642;New Ruipeng Pet Medical Group Limited,Shenzhen Guangdong 518000;Shenzhen Ruipeng Public Welfare Foundation,Shenzhen Guangdong 518000)

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]新瑞鹏宠物医疗集团有限公司,广东深圳518000 [3]深圳市瑞鹏公益基金会,广东深圳518000

出　　处：《广东畜牧兽医科技》2024年第1期38-42,81,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省高校质量工程建设项目⁃科产教融合实践教学基地(粤教高函〔2021〕29号);瑞鹏公益基金会高校青年教师(小动物临床)科研基金(RPJJ2020022)。

摘　　要：为观察抗菌肽对实验兔角膜创伤的治疗效果,试验设置A组(细菌抗菌肽组),B组(左氧氟沙星组),C组(模型对照组)。在实验兔成功构建角膜创伤模型之后,分别使用药物进行治疗,并观察相关试验指标。试验结果显示,与模型对照组比较,细菌抗菌肽组与左氧氟沙星组实验兔的泪液分泌量均显著低于模型对照组(P<0.05);在试验第28天的角膜荧光染色结果显示,抗菌肽组实验兔角膜的深层损伤已基本修复,其效果优于左氧氟沙星组;与模型对照组比较,抗菌肽组实验兔的眼房水中IL⁃1、IL⁃8、MMP⁃2等促炎因子含量水平显著降低(P<0.05),且明显低于左氧氟沙星组;活菌计数结果显示,细菌抗菌肽对实验兔角膜创伤后眼部细菌感染的抑制作用显著高于左氧氟沙星(P<0.05)。试验结果表明,抗菌肽在治疗实验兔角膜损伤的综合疗效明显优于左氧氟沙星,为细菌抗菌肽在兽医临床上治疗宠物角膜损伤的应用提供了一定的实验数据与理论依据。This study focused on the efficacy of antimicrobial peptides to corneal trauma in experimental rabbits.Three study groups were set up for the trial,including group A(bacterial antimicrobial peptide group),group B(levofloxacin group)and group C(model control group).The rabbit corneal trauma model was first established.Different treatment was applied separately and the relevant test indicators were observed.The results showed that the tear secretion of the experimental rabbits in both the bacterial antimicrobial peptide and levofloxacin treatment groups were significantly lower than that of the model control group(P<0.05).Corneal fluorescence staining on day 28 of the trial showed that deep corneal damage had been largely repaired in the rabbits’s cornea of the antimicrobial peptide treatment group,which was superior to that of the levofloxacin treatment group.Compared with the model control group,the levels of pro⁃inflammatory factors such as IL⁃1,IL⁃8 and MMP⁃2 in the aqueous humor of the experimental rabbits in the antimicrobial peptide treatment group were significantly lower(P<0.05)than those in the levofloxacin treatment group.The results of the live bacterial count showed that the bacterial antimicrobial peptide inhibited ocular infections after corneal trauma significantly more than levofloxacin in experimental rabbits(P<0.05).The results of the experiment showed that the comprehensive efficacy of the antimicrobial peptide in the treatment of corneal injury in experimental rabbits was significantly better than that of levofloxacin,which provided some experimental data and theoretical basis for the application of bacterial antimicrobial peptide in the treatment of corneal injury in pets in veterinary clinics.

关 键 词：角膜损伤 抗菌肽 兔 活菌计数 

分 类 号：S854[农业科学—临床兽医学]