胺基功能化MOF材料固定化纤维素酶在稀酸预处理液中的稳定性研究  

Stability of amine-functionalized MOF materials immobilized cellulase in dilute acid pretreatment slurry

作　　者：沈欣 严立石 SHEN Xin;YAN Lishi(School of Chemistry and Life Sciences,SUST,Suzhou 215009,China)

机构地区：[1]苏州科技大学化学与生命科学学院,江苏苏州215009

出　　处：《苏州科技大学学报（自然科学版）》2024年第1期54-60,共7页Journal of Suzhou University of Science and Technology（Natural Science Edition）

基　　金：国家自然科学基金项目(21506137);苏州市产业创新项目(SNG202044)。

摘　　要：合成一种金属有机骨架材料(Metal-organic frameworks,MOF)ZIF-8,对其进行胺基功能化获得ZIF-8-NH_(2),并通过戊二醛将纤维素酶交联在ZIF-8-NH_(2)上制备固定化纤维素酶ZIF-8-NH_(2)@Cellulase。研究表明,ZIF-8-NH_(2)的最大酶负载量(245.8 mg·g^(-1))大于ZIF-8(198.3 mg·g^(-1))。与游离纤维素酶相比,ZIF-8-NH_(2)@Cellulase的pH稳定性、温度稳定性、发酵抑制物耐受性和酶促反应效率等方面均表现出更好的性能。当香草醛和糠醛的用量都为5 g·L^(-1)时,ZIF-8-NH_(2)@Cellulase的相对酶活分别比游离酶高17.6%和6.3%。此外,ZIF-8-NH_(2)@Cellulase连续循环使用5次仍能保持70%以上的相对酶活。这意味着ZIF-8-NH_(2)@Cellulase有应用于稀酸预处理后酶解的潜力。A metal-organic framework material ZIF-8 was synthesized and amine-functionalized to obtain ZIF-8-NH_(2).Subsequently,cellulase was successfully cross-linked onto ZIF-8-NH_(2) by glutaraldehyde(ZIF-8-NH_(2)@Cellulase).It was found that the maximum enzyme loading of ZIF-8-NH_(2)(245.8 mg·g^(-1))was greater than that of ZIF-8(198.3 mg·g^(-1)).Compared with free cellulose,ZIF-8-NH_(2)@Cellulase showed better performance in terms of temperature stability,pH stability,fermentation inhibitor tolerance and enzymatic reaction efficiency.In the presence of 5 g·L^(-1) of vanillin and furfural,the activity of ZIF-8-NH_(2)@Cellulase was 17.6%and 6.3%higher than that of the free enzyme,respectively.Furthermore,ZIF-8-NH_(2)@Cellulase maintained more than 70%relative enzyme activity after 5 reuses.This study demonstrated that ZIF-8-NH_(2)@Cellulase has the potential to be applied to enzymatic hydrolysis after dilute acid pretreatment.

关 键 词：金属有机骨架材料 载体修饰 固定化纤维素酶 

分 类 号：Q815[生物学—生物工程]