亚洲百合‘穿梭’组培快繁体系的建立  

Establishment of Lilium‘Trensor’in vitro Rapid Propagation System

作　　者：马秀明 胡云超 缪军[1] 张庶[1] 王俊峰[1] 石少川 MA Xiuming;HU Yunchao;MIAO Jun;ZHANG Shu;WANG Junfeng;SHI Shaochuan(Institute of Vegetable,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100;Jinan Zhongke Green Biological Engineering Co.Ltd.,Jinan,Shandong 250100)

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]济南中科绿色生物工程有限公司,山东济南250100

出　　处：《北方园艺》2024年第4期42-48,共7页Northern Horticulture

基　　金：山东省农业科学院农业科技创新工程资助项目(CXGC2023F08)。

摘　　要：以亚洲百合‘穿梭’为试材,采用植物组织培养的方法,研究了不同浓度激素对百合鳞片诱导、不定芽增殖、小鳞茎生根培养的影响,并筛选出适宜百合组培苗生长的移栽基质,以期为亚洲百合‘穿梭’的组培快繁体系的建立提供参考依据。结果表明:百合鳞片最佳诱导培养基为MS+0.5 mg·L^(-1)NAA+0.5 mg·L^(-1)6-BA+30 g·L^(-1)蔗糖+6 g·L^(-1)琼脂,诱导率最高可达74.44%,显著高于其他处理;最佳增殖培养基为MS+0.1 mg·L^(-1)NAA+1.0 mg·L^(-1)6-BA+30 g·L^(-1)蔗糖+6 g·L^(-1)琼脂,不定芽增殖数量最多,增殖系数为3.27;适合小鳞茎生根培养基为1/2MS+0.1 mg·L^(-1)NAA+0.3 mg·L^(-1)IBA+40 g·L^(-1)蔗糖+6 g·L^(-1)琼脂,平均生根数量为14.33,是其他处理的1.15~2.02倍;移栽后组培苗在草炭与珍珠岩为1∶1的基质配比中成活率高达87.50%,且组培苗长势最好。The‘Trensor’of Asiatic lily was used as the test material.The effects of different concentrations of hormones on the scale induction,adventitious bud proliferation and bulb rooting culture of Asiatic lily were studied by the method of plant tissue culture,and the effects of different transplanting substrates on the growth of lilium tissue culture seedlings were studied,in order to provide reference for the establishment of‘Trensor’tissue culture rapid propagation system of Lilium asiatica.The results showed that the optimal medium for lilium scale induction was MS+0.5 mg·L^(-1) NAA+0.5 mg·L^(-1)6-BA+30 g·L^(-1) sucrose+6 g·L^(-1) agar,and the highest induction rate was 74.44%,which was higher than other treatments.The best proliferation medium was MS+0.1 mg·L^(-1) NAA+1.0 mg·L^(-1)6-BA+30 g·L^(-1) sucrose+6 g·L^(-1) agar,and the number of advective buds was the highest with a proliferation coefficient of 3.27.The suitable rooting medium was 1/2MS+0.1 mg·L^(-1) NAA+0.3 mg·L^(-1) IBA+40 g·L^(-1) sucrose+6 g·L^(-1) agar,and the average rooting number was 14.33.It was 1.15-2.02 times more than other treatments.After transplanting,the survival rate of tissue culture seedlings was 87.50%in the matrix ratio of peat and perlite was 1∶1,and the growth of tissue culture seedlings was the best.

关 键 词：植物激素 组织培养 快繁体系 

分 类 号：S682.29[农业科学—观赏园艺]