灵芝菌株发菌期对玉米秸秆中木质纤维素的利用  

Utilization of Lignocellulose From Maize Stalk by Ganoderma lingzhi Strain

作　　者：王胜 李金涛 闫梅霞[1,2] WANG Sheng;LI Jintao;YAN Meixia(Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun,Jilin 130112;Jilin Provincial Key Laboratory of Traditional Chinese Medicinal Materials Cultivation and Propagation,Changchun,Jilin 130112)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]吉林省中药材种植(养殖)重点实验室,吉林长春130112

出　　处：《北方园艺》2024年第4期96-103,共8页Northern Horticulture

基　　金：中国农业科学院科技创新工程资助项目(CAAS-ASTIP-2021-ISAPS)。

摘　　要：以4株灵芝菌株为试材,采用PDA-愈创木酚培养基、PDA-苯胺蓝培养基、产纤维素酶筛选性培养基、产木聚糖酶筛选性培养基对这些菌株进行产木质纤维素酶能力的初筛,研究了菌株发菌期不同培养料配方中的菌丝生长速率、木质纤维素降解酶活性及其对玉米秸秆木质纤维素的降解,以期为研究灵芝发菌期灵芝利用木质纤维素的规律提供参考依据。结果表明:利用4种培养基筛选出木质纤维素酶活性较高的LZ-10,并用于后续试验。玉米秸秆降解试验中,随着配方1~7中玉米秸秆占比的增加,菌丝生长速度呈现先增加后减小的趋势,其中配方1达到最大值(5.72±0.12)mm·d^(-1)。在灵芝发菌期内,不同配方间木质纤维素酶活性与木质纤维素降解率之间存在差异。在配方5、6中,木质素的降解率最大达到43.08%±0.05%,漆酶和锰过氧化物酶活性最大。木聚糖酶活性最大的配方5、6中,半纤维素的降解率最大达到36.20%±0.23%,而纤维素的降解率变化不明显。综上所述,灵芝发菌期内玉米秸秆添加量为80%~90%时菌丝生长较为缓慢,但对木质纤维素的利用较为全面。Four Ganoderma lingzhi strains were used as test materials,PDA-guaiacol medium,PDA-aniline blue medium,cellulase-producing screening medium and xylanase-producing screening medium were used to determine the production capacity of LCC in these strains.The mycelial growth rate,the activity of lignocellulose degrading enzyme and the degradation of lignocellulose in corn stalk were studied in different cultures during the bacterial development stage,in order to provide reference for the study of lignocellulose utilization in Ganoderma lingzhi during the bacterial development stage.The results showed that LZ-10 with high Lignocellulase activity was screened by 4 kinds of medium and used in the follow-up experiment.In the degradation experiment of corn straw,with the increase of the proportion of corn straw in formula 1 to 7,the mycelial growth rate showed a trend of first increasing and then decreasing,in which formula 1 reached the maximum value of(5.72±0.12)mm·d-1.There were differences in lignocellulosic enzyme activity and lignocellulosic degradation rate among different formulations during the growth period of Ganoderma lingzhi.In formulations 5 and 6,the degradation rate of lignin reached 43.08%±0.05%,and the activities of laccase and manganese peroxidase were the highest.In formulations 5 and 6 with the highest xylanase activity,the maximum degradation rate of hemicellulose reached 36.20%±0.23%,while the degradation rate of cellulose did not change significantly.In summary,the mycelial growth was slow when the added amount of corn stalk was 80%-90% during the period of Ganoderma lingzhi development,but the utilization of lignocellulose was more comprehensive.

关 键 词：玉米秸秆 筛选 灵芝 木质纤维素 木质纤维素酶 

分 类 号：S567.31[农业科学—中草药栽培]