健脾止动汤对抽动障碍模型大鼠纹状体快速放电中间神经元的影响  

Effection of Jian-Pi-Zhi-Dong Decoction on Fast-spiking Interneurons in Striatum of TD Rats

作　　者：陈宏[1] 王素梅[2] 卫利[2] 姜冰 印雷 黄训言 张瑞婧 CHEN Hong;WANG Sumei;WEI Li(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078 [3]中医杂志社,北京100700

出　　处：《四川中医》2023年第12期73-77,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家青年科学基金项目(编号:81202725);国家中医药管理局委托办事经费(编号:040105002001)。

摘　　要：目的:对亚氨基二丙腈(IDPN)诱导的抽动障碍(TD)大鼠模型的行为学特征进行观察,并测定其纹状体中的快速放电中间神经元(FSIs)水平,揭示TD可能的发生机制,也为健脾止动汤治疗抽动障碍提供神经解剖学证据。方法:36只雄性SD大鼠,根据体重随机分为4组,即模型组、盐酸硫必利组、健脾止动汤组和空白对照组。除空白对照组外,其余3组采用IDPN腹腔注射建立TD大鼠模型,造模成功后,进行刻板运动评分及灌胃给药,西药组给予盐酸硫必利混悬液,中药组给予健脾止动汤,空白对照组以及模型组给予生理盐水,连续给药4周后,再进行刻板运动评分以及体重的测量,并通过免疫组化法分析各组大鼠纹状体FSIs的数量以及平均光密度值。结果:(1)体重:模型组较空白组大鼠体重显著减轻(P<0.01)。灌胃4周末,健脾止动汤组大鼠体重较模型组增加(P<0.05)。(2)刻板运动评分:造模第7日,模型组大鼠评分均大于1分,提示造模成功。灌胃4周末,盐酸硫必利组、健脾止动汤组的评分均较模型组降低(P<0.05),但两组间评分无明显差异(P>0.05)。(3)PV阳性神经元数目:模型组大鼠脑纹状体的PV阳性神经元数量比空白组显著减少(P<0.01)。健脾止动汤组大鼠脑纹状体的PV阳性神经元个数多于模型组(P<0.05),盐酸硫必利组与健脾止动汤组大鼠脑纹状体PV阳性神经元个数比较,差异无统计学意义(P>0.05)。(4)平均光密度:模型组大鼠脑纹状体PV阳性神经元平均光密度值比空白组显著减少(P<0.01)。健脾止动汤组大鼠脑纹状体PV阳性神经元平均光密度值多于模型组(P<0.05),盐酸硫必利组与健脾止动汤组大鼠脑纹状体PV阳性神经元平均光密度值比较,差异无统计学意义(P>0.05)。结论:纹状体PV阳性神经元减少可能参与TD大鼠的抽动行为,健脾止动汤通过维持或修复受损的PV阳性神经元,恢复其数量和表达强度,从而改善大鼠的抽动Objective:We detect the changes of FSIs contents in striatum of TD rats induced by IDPN by immunohistochemistry,in order to provide neuroanatomical evidence of treating TD for Jian-Pi-Zhi-Dong Decoction.Methods:36 male SD rats were randomly divided into 4 groups according to body weight,namely model group,tiapride hydrochloride group,Jian-Pi-Zhi-Dong Decoction group and blank control group,with 9 rats in each group.In addition to the blank control group,the other three groups were injected intraperitoneally with IDPN(250mg/kg/D)to establish TD rat models.After successful modeling,the stereotyped exercise score and intragastric administration were carried out.The western medicine group was given tiapride hydrochloride suspension(21mg/kg/D),and the traditional Chinese medicine group was given Jian-Pi-Zhi-Dong Decoction(16mg/kg/D),and the blank control group and the model group were given normal saline(10mg/kg/D).After continuous administration for 4 weeks,the stereotyped exercise score and body weight were measured.The number and average optical density of FSIS in striatum of rats in each group were analyzed by immunohistochemistry.Results:①body weight:the body weight of the model group was significantly lower than that of the blank group(P<0.01).At the end of 4 weeks after gavage,the body weight of rats in Jian-Pi-Zhi-Dong Decoction group was higher than that in model group(P<0.05).②Stereotyped motor score:on the 7th day of modeling,except for the blank group,if the score of each animal in each group was greater than 1 point,then the modeling was successful.At the end of 4 weeks after gavage,the score of tiapride hydrochloride group was lower than that of model group(P<0.05),and that of Jian-Pi-Zhi-Dong Decoction group was lower than that of model group(P<0.05),but there was no significant difference between tiapride hydrochloride group and Jian-Pi-Zhi-Dong Decoction group(P>0.05).③Number of PV positive neurons:the number of PV positive neurons in the striatum of the model group was significantly lower than

关 键 词：抽动障碍模型 亚氨基二丙腈 健脾止动汤 快速放电中间神经元 PV阳性神经元 纹状体 

分 类 号：R285.5[医药卫生—中药学]