烟草Nt-GAD的氨基酸序列分析  

Amino Acid Aequence Analysis of Nt-GAD in Tobacco

作　　者：龙岗 陈树鸿 龙建林 高萍 赵娟 LONG Gang;CHEN Shuhong;LONG Jianlin;GAO Ping;ZHAO Juan(Huili Branch,Liangshan Prefecture Company,Sichuan Tobacco Corporation,Huili,Sichuan 615100,China)

机构地区：[1]四川省烟草公司凉山州公司会理分公司,四川会理615100

出　　处：《陕西农业科学》2024年第1期7-10,105,共5页Shaanxi Journal of Agricultural Sciences

摘　　要：为从分子层面研究烟草Nt-GAD的氨基酸序列,采用Protparam、Interpro、Blast、Muscle、Phyml和Treedyn工具,分析烟草Nt-GAD氨基酸序列的理化特性、结构域和进化树。结果表明:烟草Nt-GAD成员的等电点均处于5.5~6之间,且Nt-GAD1和Nt-GAD2同源性最高,此外Nt-GAD2的耐热性最好,Nt-GAD3的稳定性最强;烟草Nt-GAD属于磷酸吡哆醛依赖性脱羧酶家族和谷氨酸脱羧酶家族,且具有磷酸吡哆醛依赖性转移酶同源超家族结构域,C末端存在独特区域钙调蛋白结构域,通过进化树分析烟草Nt-GAD成员被划分为三组,其中烟草Nt-GAD3(第一组)分化较为突出,而Nt-GAD2(第三组)则较为保守,而Nt-GAD1和Nt-GAD4被划分为第二组。To investigate the amino acid sequence of tobacco Nt-GAD at the molecular level, the physical and chemical characteristics, structural domains and evolutionary trees of tobacco Nt-GAD amino acid sequence were analyzed using the tools of Protparam, Interpro, Blast, Muscle, Phyml and Treedin. The results showed that the isoelectric points of tobacco Nt-GAD members ranged between 5.5~6, and Nt-GAD1 exhibited the highest homology with Nt-GAD2 . In addition, Nt-GAD2 had the best heat resistance and Nt-GAD3 had the strongest stability. Tobacco Nt-GAD was classified within the pyridoxal phosphate-dependent decarboxylase family and the glutamate decarboxylase family, possessing the pyridoxal phosphate-dependent transferases homologous superfamily domain. A distinctive calmodulin domain was located at the C-terminal. Based on evolutionary tree analysis, tobacco Nt-GAD members were categorized into three groups.Among these group, tobacco Nt-GAD3 (group 1) is more prominent, whereas Nt-GAD2 (group 3) is more conservative, Nt-GAD1 and Nt-GAD4 constitute the second group.

关 键 词：烟草 谷氨酸脱羧酶 生物学 

分 类 号：S511[农业科学—作物学]