烟草苗期立枯病病原菌Rhizoctonia solani AG-1 IB全基因组序列资源  

Complete genome sequence resource for Rhizoctonia solani AG-1 IB causing sore shin in tobacco seedlings

作　　者：向立刚 蔡刘体 李文红[3] 余知和[4] 汪汉成 穆青 XIANG Ligang;CAI Liuti;LI Wenhong;YU Zhihe;WANG Hancheng;MU Qing(College of Agriculture,Yangtze University,Jingzhou 434025,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;Guizhou Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;College of Life Sciences,Yangtze University,Jingzhou 434025,China;Qianxi′nan Zhou Company of Guizhou Tobacco Company,Xingyi 562400,China)

机构地区：[1]长江大学农学院,荆州434025 [2]贵州省烟草科学研究院,贵阳550081 [3]贵州省农业科学院植物保护研究所,贵阳550006 [4]长江大学生命科学学院,荆州434025 [5]贵州省烟草公司黔西南州公司,兴义562400

出　　处：《植物病理学报》2023年第6期1027-1037,共11页Acta Phytopathologica Sinica

基　　金：National Natural Science Foundation of China(31960550,32160522);The“Hundred”Level Innovative Talent Foundation of Guizhou Province(GCC[2022]028-1);China National Tobacco Corporation(110202101048(LS-08),110202001035(LS-04));Guizhou Science Technology Foundation(ZK[2021]Key036);Qianxinan Zhou Company of Guizhou Tobacco(2021-06);Guizhou Tobacco Company(2020XM03,2020XM22)。

摘　　要：立枯丝核菌(Rhizoctonia solani)AG-1 IB融合群是重要的土传病原真菌,可引起烟草苗期立枯病。本研究通过Illumina HiSeq PE 150和Oxford Nanopore PromethION测序平台获得了分离自发病烟草植株的R.solani AG-1 IB LK4菌株的全基因组序列。结果表明,该基因组全长45.9 Mbp,包含112个重叠群。重叠群的N50为0.89 Mbp,GC含量为52.13%。基因组共线性分析结果显示,LK4与其他4个已公开发布基因组序列的R.solani AG-1 IB菌株相比,易位和易位+反转现象更为常见,表明LK4菌株在基因组结构上具有独特性。LK4基因组中共预测到7,978个蛋白编码基因,231个非编码基因以及5,191个重复序列。NR、GO、KEGG、Swiss-Prot、COG、PHI、Signal P、CAZy、DFVF、TCDB和P450数据库被用于蛋白编码基因的分类与注释,其中有7,375个蛋白编码基因至少被上述数据库注释过一次。该基因组的组装和注释对进一步揭示立枯丝核菌的致病机理以及烟草立枯病的防控研究具有重要意义。Rhizoctonia solani AG-1 IB is an important soil-borne fungal pathogen that causes sore shin of tobacco seedlings.In this study,we obtained a new 45.9 Mbp genome sequence of R.solani AG-1 IB strain LK4 isolated from diseased tobacco seedlings using Illumina HiSeq PE 150 and Oxford Nanopore PromethION platforms.The genome consists of 112 contigs with a N50 value of 0.89 Mbp and a GC content of 52.13%.Results of genome synteny analysis showed that translocation and translocation+inversion are more common in strain LK4 as compared with those in the genome sequences of other four publicly-released R.solani AG-1 IB strains,suggesting that strain LK4 is different from the four strains in genome structure.A total of 7,978 protein-coding genes,231 no-coding RNAs,and 5,191 repeat sequences were predicted.The NR,GO,KEGG,Swiss-Prot,COG,PHI,Signal P,CAZy,DFVF,TCDB,and P450 databases were used to annotate and categorize protein-coding genes,and 7,375 protein-coding genes were annotated at least once.This new genome assembly and annotation resource will help elucidate the pathogenic mechanism of R.solani AG-1 IB and improve the management of tobacco sore shin disease.

关 键 词：立枯丝核菌 融合群 基因组序列 基因功能 立枯病 烟草 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]