浙贝母芽枝霉斑病病原菌鉴定、生物学特性及生物药剂防治效果研究  

Identification biological characteristics,and inhibition efficacy of plant-derived antifungal agents of pathogen Cladosporium spp.causing mildew spot of Fritillaria thunbergii

作　　者：龙安琪 朱泽 李强 郭俭 何晓婵 朱丽燕 周小军 李玲[1] LONG Anqi;ZHU Ze;LI Qiang;GUO Jian;HE Xiaochan;ZHU Liyan;ZHOU Xiaojun;LI Ling(College of Advanced Agricultural Sciences,Zhejiang Agriculture and Forest University,Hangzhou 311300,China;Jinhua Academy of Agricultural Sciences,Jinhua 321000,China;College of Food and Health,Zhejiang Agriculture and Forest University,Hangzhou 311300,China)

机构地区：[1]浙江农林大学现代农学院,杭州311300 [2]金华市农业科学研究院,金华321000 [3]浙江农林大学食品与健康学院,杭州311300

出　　处：《植物病理学报》2023年第6期1047-1055,共9页Acta Phytopathologica Sinica

基　　金：磐安县中药材绿色发展示范项目(磐中药发202005);金华市农业类重大项目“基于道地性评价的中药材生态种植技术研究”(计划编号:2019-2-001a)。

摘　　要：浙贝母(Fritillaria thunbergii Miq.)作为浙江省地道中药材,属“浙八味”之一。近年来在磐安县浙贝母主产区发生一种新的霉斑病,发病部位有褐色病斑,潮湿时产生大量霉层,严重影响浙贝母的生长与发育。本研究利用病原菌分离与鉴定、致病性测定、生物学形态测定及ITS、ACT和EF-α多基因序列分析等方法,鉴定出浙贝母芽枝霉斑病的病原菌为Cladosporium pseudocladosporioides。该病原菌菌丝生长的最适温度为25℃,最适pH值为7~8,以麦芽糖为最适碳源,牛肉浸膏和胰蛋白胨为最适氮源。本文同时还进行了3种不同生物药剂对该病原菌的室内毒力测定,发现0.5%小檗碱水剂对其抑制效果最好。本研究为浙贝母芽枝霉斑病识别与防治提供了科学依据。Fritillaria thunbergii Miq.is one of the traditional Chinese medicines in Zhejiang Province.In recent years,a new mildew spot disease has occurred in the main producing area of F.thunbergii of Pan′an County.The large amount of mold layer appeared on the surface of diseased stems and leaves seriously affecting the growth and development of F.thunbergii.In this study,the pathogen was isolated and the two purified cultures YZ-1 and YZ-3 were obtained,which caused the typical symptoms similar to that in the field by the pathogenicity test assay.Combined with the biological morphology which was obviously similar to Cladosporium spp.and the analysis of sequence set of ITS,ACT and EF-αgene,isolates YZ-1 and YZ-3 was identified as Cladosporium pseudocladosporioides,the pathogen of mildew spot disease of Fritillaria thunbergii.The optimum temperature and pH for mycelial growth of the pathogen were at 25℃and value 7-8,respectively.Maltose was the optimum carbon source,and beef extract and tryptone were the optimum nitrogen sources.In this paper,the toxicity of three different plant-derived antifungal agents to the pathogen was also determined in laboratory.It was found that 0.5%of berberine aqueous solution exhibited the best inhibitory effect on the pathogen growth on PDA plates.This study provides a scientific basis for the identification and prevention of Cladosporium mildew of Fritillaria thunbergii.

关 键 词：浙贝母 Cladosporium pseudocladosporioides 序列分析 生物学特性 生物防治 

分 类 号：S432.44[农业科学—植物病理学]