小麦黄花叶病品种抗性与根部禾谷多黏菌数量的关系分析  

Relationship between the amount of Polymyxa graminis in root and wheat cultivar’s resistance to wheat yellow mosaic virus

作　　者：林佳宇 常青 孙航军 李永辉 李洪连[1] 孙炳剑[1] LIN Jiayu;CHANG Qing;SUN Hangjun;LI Yonghui;LI Honglian;SUN Bingjian(College of Plant Protection,Henan Agricultural University,Zhengzhou 450046)

机构地区：[1]河南农业大学植物保护学院,郑州450046

出　　处：《植物病理学报》2023年第6期1266-1271,共6页Acta Phytopathologica Sinica

基　　金：国家自然基金国际(地区)合作交流项目(31861143008);河南农业大学作物学科合作研究基金(CCSR2022-2);河南省重大公益专项(201300111600)。

摘　　要：0引言小麦黄花叶病是我国冬麦区最重要的病毒病害,其病原是小麦黄花叶病毒(wheat yellow mosaic virus,WYMV)[1],可引起小麦叶片黄化,分蘖减少,株型矮缩,后期麦穗短小,发育不良[2],一般造成小麦减产10%~30%,严重的达70%以上[3]。自20世纪90年代以来,该病害每年发生面积都在66.7万公顷以上,主要分布在我国河南、江苏、山东、安徽、陕西、湖北、四川等省,呈逐年蔓延加重趋势[3-4]。Wheat yellow mosaic virus(WYMV)disease,the most important viral disease in the winter wheat region of China,is more and more serious in recent years.The pathogen,WYMV,is transmitted by Polymyxa graminis.Investigating the connection between wheat cultivar’s resistance to WYMV and the amount of P.graminis in root is instructive,as it is beneficial to the understanding of the mechanism of wheat cultivar’s resis-tance and can offer guidance for the breeding of cultivars.Based on the rDNA of P.graminis,a pair of specific primers were designed.The SYBR Green I real-time PCR reaction system was established and optimized.We evaluated 70 wheat cultivars according to their disease severity in field disease nursery and examined whether P.graminis existed in their root using PCR.Four cultivars,which exhibited uniform disease severity,were chosen from a collection of cultivars with similar disease severity,and the amounts of P.graminis in their roots were determined using real-time PCR.Results showed that among the 70 cultivars,32 exhibited level 0 of disease severity,9 exhibited level 1,7 exhibited level 2 and 22 exhibited level 3.P.graminis was identified from the root of all these 70 cultivars using PCR.Real-time PCR showed that the wheat cultivar that exhibited higher disease severity had a greater number of P.graminis in their root.The disease severity of wheat cultivar presented a positive relationship with the amount of P.graminis in root.The wheat cultivars that exhibited level 3 of disease severity had the greatest number of P.graminis in their root.But,some wheat cultivars that exhibited level 0 of disease severity had a greater number of P.graminis in their root.These results indicated that the resistance of wheat cultivar to WYMV was related to the resistance to P.graminis in their root.

关 键 词：小麦黄花叶病 病毒病害 叶片黄化 发育不良 品种抗性 冬麦区 公项 20世纪90年代以来 

分 类 号：S432.1[农业科学—植物病理学]