内蒙古包头市部分规模化牛场BRV流行病学调查  

Epidemiological Investigation of BRV in Some Large-scale Cattle Farms in Baotou City of Inner Mongolia

作　　者：陈超越 杭天宇 郭宇 谢梦圆 石晓娇 徐晓静[1] CHEN Chaoyue;HANG Tianyu;GUO Yu;XIE Mengyuan;SHI Xiaojiao;XU Xiaojing(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot O10018,China;Animal Health and Slaughtering Management Station in Hengshan District,Yulin 719000,China;Inner Mongolia Animal Disease Control Center,Hohhot 010020,China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]陕西省榆林市横山区动物卫生与屠宰管理站,陕西榆林719000 [3]内蒙古自治区动物疫病预防控制中心,内蒙古呼和浩特010020

出　　处：《兽医导刊》2023年第3期20-22,共3页Veterinary Orientation

基　　金：呼和浩特市重大科技专项(2022-农-重-1-1);呼和浩特市科技创新领域人才项目(2022RC-联合体-1);内蒙古自治区科技重大专项“牛腹泻疾病防控关键技术研发与标准化操作规程示范推广”(2020ZD0006);内蒙古自治区科技重大专项“牛羊支原体肺炎和病毒性呼吸道疾病的防控技术研发与示范”(2021ZD0013)。

摘　　要：为了解内蒙古自治区包头市部分规模化牛场牛轮状病毒(BRV)的流行情况,在包头市部分规模化牛场采集275份血液样本和300份粪便样本,采用RT-PCR和ELISA方法对血液样本和粪便样本进行牛轮状病毒抗原和抗体检测。结果显示,BRV抗原总阳性率为21.34%,抗体总阳性率为4.00%。在检测样品中,0~2月龄犊牛的抗原阳性率和抗体阳性率均最高,分别为51.00%和10.00%,本研究检测结果可为规模化牛场牛轮状病毒的防控提供参考。In order to understand the prevalence of bovine rotavirus in some large-scale cattle farms in Baotou City,Inner Mongolia Autonomous Region,275 blood samples and 300 stool samples were collected.RT-PCR and ELISA were used to detect bovine rotavirus(BRV)antigen and antibody in blood samples and stool samples.The results of the study showed that the total positive rate of BRV antibody was 21.34%and the total positive rate of antigen was 4%.Among the samples tested,the antibody positive rate and antigen positive rate of 0~2 month old calves were the highest,which were 51.00%and 10.00%,respectively.The results of this study provide reference for the prevention and control of rotavirus disease in large-scale cattle farms.

关 键 词：牛轮状病毒 流行病学调查 ELISA RT-PCR 

分 类 号：S852.65[农业科学—基础兽医学]