阿拉善盟骆驼布鲁氏菌病血清学检测方法的比较  

Comparison of Serological Testing Methods for Camel Brucellosis in Alxa League

作　　者：高登军 郭宇 陈广林 苏晓玲 林立杰 特木尔巴根 GAO Dengjun;GUO Yu;CHEN Guanglin;SU Xiaoling;LIN Lijie;Temuerbagen(Inner Mongolia Animal Disease Control Center,Hohhot O10020,China;Inner Mongolia Alashan Youqi Animal Disease Control Center,Alashan 737300,China)

机构地区：[1]内蒙古自治区动物疫病预防控制中心,内蒙古呼和浩特010020 [2]内蒙古阿拉善盟阿拉善右旗动物疫病预防控制中心,内蒙古阿拉善737300

出　　处：《兽医导刊》2023年第3期23-26,共4页Veterinary Orientation

摘　　要：骆驼是布鲁氏菌病病原体的重要携带者和感染源之一,骆驼布病的检测至关重要。通过虎红平板凝集试验(RBT)和荧光偏振方法(FPA)两种方法对已知感染的阿拉善盟102峰骆驼血清样本进行检测,结果RBT方法检测表明90份样本呈阴性,12份阳性;FPA检测表明102份样本全部为阴性;表明RBT检测骆驼布病假阳性率较高,FPA法准确可靠。建议在临床诊断中选用RBT作为骆驼布病的初筛检测方法,对初筛检测结果为阳性的样本,结合临床症状,采用FPA进行复核。Camels have become one of the important carriers and infection sources of brucellosis pathogens.The detection of camel brucellosis was crucial.In this study,102 camel samples collected from Alxa League,Inner Mongolia were tested by RBT and FPA.The results showed that 90 samples were negative and 12 were positive in RBT.All 102 samples in the FPA were negative.The false positive rate of RBT is higher than that of FPA,which is accurate and reliable.In clinical diagnosis,RBT is recommended as the primary screening detection method for camel brucellosis.For samples with positive preliminary screening results,FPA is used for review combined with clinical symptoms.

关 键 词：骆驼 布鲁氏菌病 虎红平板凝集试验 荧光偏振方法 检测 

分 类 号：S858.24[农业科学—临床兽医学]