瓜蒌提取物对缺血性心肌病大鼠心肌纤维化改善作用的实验研究  

Experimental Study on Improvement Effects of Gualou(Trichosanthes)Extract on Myocardial Fibrosis in Rats with Ischemic Cardiomyopathy

作　　者：王巍 卢凯 诸帆[1] 章琪[1] WANG Wei;LU Kai;ZHU Fan;ZHANG Qi(Department of Cardiovascular Medicine,The First People’s Hospital of Huzhou,Huzhou,Zhejiang 313000)

机构地区：[1]湖州市第一人民医院心血管内科,浙江湖州313000

出　　处：《中国中医药科技》2024年第2期214-217,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省医药卫生科技计划项目(2023KY320)。

摘　　要：目的:观察瓜蒌提取物对缺血性心肌病(ICM)大鼠心肌纤维化的改善作用,并探讨其作用机制。方法:SD大鼠36只,随机分成正常对照组、模型组、瓜蒌提取物组,每组12只。腹腔注射20%氨基甲酸乙酯(5 mg/kg)制备大鼠ICM模型,瓜蒌提取物组灌胃瓜蒌提取物溶液4 g/kg,正常对照组和模型组灌胃相同剂量生理盐水,给药4周,ELISA法检测血清心肌纤维化指标[转化生长因子(TGF)-1β、结缔组织生长因子(CTGF)、半乳糖凝集素(Gal)-3],化学发光法检测氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)],ELISA法检测炎性因子[白细胞介素(IL)-6,肿瘤、坏死因子(TNF)-α,C反应蛋白(CRP)]。光镜观察各组大鼠心肌组织病理变化。结果:模型组血清TGF-1β、CTGF、Gal-3水平均高于正常组,瓜蒌提取物组TGF-1β、CTGF、Gal-3水平均低于模型组,差异有统计学意义(P<0.05)。模型组血清SOD活性低于正常组,MDA含量高于正常组;瓜蒌提取物组SOD活性高于模型组,MDA含量低于模型组,差异有统计学意义(P<0.05)。模型组血清TNF-α、IL-6、CRP水平高于正常组,瓜蒌提取物组TNF-α、IL-6、CRP水平低于模型组,差异有统计学意义(P<0.05)。Masson染色显示,正常组大鼠心肌组织血管周围见少量均匀分布的胶原纤维。模型组大鼠心肌组织可见大量胶原纤维,且深入组织间隙,分布不均匀,形态杂乱。瓜蒌提取物组心肌组织形态基本正常。结论:瓜蒌提取物能够减轻ICM大鼠心肌纤维化程度,其作用与抗氧化、抗炎作用有关。Objective:To observe the effect of Trichosanthes Extract on myocardial fibrosis in rats with ischemic cardiomyopathy(ICM)and explore its mechanism.Methods:36 male SD rats of clean grade were randomly divided into normal control group,model group and Trichosanthes Extract group with each group 12 rats.The rat model was prepared by intraperitoneal injection of 20%ethyl carbamate(5 mg/kg).Trichosanthis Extract group was intragaically irrigated with Trichosanthis Extract solution 4 g/kg,and normal control group and model group were intragaically irrigated with the same dose of normal saline for 4 weeks.Myocardial fibrosis indexes[transforming growth factor(TGF)-1β,connective tissue growth factor(CTGF),galactoagglutinin(Gal)-3]were detected by ELISA,serum oxidative stress indexes[superoxide dismutase(SOD),malondialdehyde(MDA)]were detected by chemiluminescence method,serum inflammatory factors[interleukin(IL)-6,tumor,necrosis factor(TNF)-α,C-reactive protein(CRP)]were detected by ELISA.The pathological changes of myocardial tissue in each group were observed by light microscope.Results:The levels of serum TGF-1β,CTGF and Gal-3 in model group were higher than those in the normal group,the levels of TGF-1β,CTGF and Gal-3 in the Trichosanthes Extract group were lower than those in the model group,and the diffe-rences were statistically significant(P<0.05).The serum SOD activity in the model group was lower than that of the normal group,and the MDA content was higher than that of the normal group,the serum SOD activity of the Trichosanthes Extract group was higher than that of the model group,and the MDA content was lower than that of the model group,the differences were statistically significant(P<0.05).The levels of serum TNF-α,IL-6 and CRP in the model group were higher than those in the normal group,the levels of TNF-α,IL-6 and CRP in the Trichosanthes Extract group were lower than those in the model group,and the differences were statistically significant(P<0.05).Masson staining showed that a small number of un

关 键 词：缺血性心肌病 心肌纤维化 瓜蒌提取物 氧化应激 炎症反应 大鼠 

分 类 号：R285.5[医药卫生—中药学]