基于血管内皮生长因子变化探讨姜味草改善阿霉素肾病大鼠蛋白尿的作用机制  

Exploring on Mechanism of Jiangweicao(Micromeria Biflora)in Reducing Proteinuria of Doxorubicin-Induced Nephropathy Rats based on VEGF Change

作　　者：雷仪玲 邬政付 张琳 崔桂仙 卜雪 LEI Yiling;WU Zhengfu;ZHANG Lin;CUI Guixian;BU Xue(The Second Clinical Medical School of Guizhou University of Chinese Medicine,Guiyang,Guizhou 550000;The Second Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang,Guizhou 550000)

机构地区：[1]贵州中医药大学第二临床医学院,贵州贵阳550000 [2]贵州中医药大学第二附属医院肾内科,贵州贵阳550000

出　　处：《中国中医药科技》2024年第2期223-226,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：贵州中医药大学大学生创新创业训练计划项目[贵中医大创合字(2021)15号]。

摘　　要：目的:观察姜味草对阿霉素肾病大鼠蛋白尿的影响,并探讨其作用机制。方法:40只大鼠分为正常对照组、模型组、中药组、西药组,采用阿霉素尾静脉注射法建立阿霉素肾病大鼠模型。造模2周后正常对照组及模型组予生理盐水灌胃,中药组及西药组分别予姜味草900 mg/(kg·d)及盐酸贝那普利0.9 mg/(kg·d)灌胃,连续给药4周,检测各组大鼠24 h尿蛋白定量(24 h TP)、血液尿素氮(BUN)、肌酐(SCr)、白蛋白(ALB)、血浆血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)及大鼠肾组织VEGF含量。结果:模型组、中药组及西药组24 h TP较正常对照组均显著升高,给药14 d、28 d,中药组及西药组24 h TP较模型组明显降低(P<0.05),且中药组低于西药组(P<0.05);模型组、中药组及西药组ALB均低于正常对照组(P<0.01),中药组ALB高于西药组(P<0.05),西药组ALB高于模型组(P<0.05)。各组间BUN及SCr比较,差异均无统计学意义(P>0.05)。模型组、中药组及西药组大鼠血浆及肾组织VEGF较正常对照组均显著升高(P<0.05),中药组血浆及肾组织VEGF低于西药组(P<0.05),西药组血浆及肾组织VEGF低于模型组(P<0.05)。结论:姜味草具有减少阿霉素肾病大鼠蛋白尿作用,其机制可能与下调肾病大鼠血浆及肾组织VEGF的表达有关。Objective:To observe the effect of Micromeria biflora on proteinuria in rats with doxorubicin-induced nephropathy and explore its mechanism.Methods:40 rats were divided into normal control group,model group,Chinese medicine group and Western medicine group.Injecting doxorubicin on tail vein one-time to establish doxorubicin nephropathy rat models.Two weeks after modeling,the normal control group and the model group were given normal saline by gavage,and the Chinese medicine group and the Western medicine group were given Micromeria biflora 900 mg/(kg·d)and Benazepril hydrochloride 0.9 mg/(kg·d)by gavage respectively for 4 weeks.The 24 h urinary protein(24 h TP),blood urea nitrogen(BUN),serum creatinine(SCr),albumin(ALB),the vascular endothelial growth factor(VEGF)content in plasma and kidney tissues were detected.Results:The 24 h TP of model group,Chinese medicine group and Western medicine group was significantly higher than that of normal control group.After administration for 14 days and 28 days,24 h TP in Chinese medicine group and Western medicine group was significantly lower than that of model group(P<0.05),and that of the Chinese medicine group was lower than that of the Western medicine group(P<0.05).The ALB content of model group,Chinese medicine group and Western medicine group was lower than that of normal control group(P<0.01),the ALB content of Chinese medicine group was higher than that of Western medicine group(P<0.05),and the ALB comtent of Western medicine group was higher than that of model group(P<0.05).There were no significant differences in BUN and SCr among all groups(P>0.05).VEGF expression in plasma and kidney tissue of model group,Chinese medicine group and Western medicine group was significantly higher than that of normal control group(P<0.05),VEGF expression in the Chinese medicine group was lower than that in the Western medicine group(P<0.05),VEGF expression in the Western medicine group was lower than that in the model group(P<0.05).Conclusion:Micromeria biflora can relieve prot

关 键 词：姜味草 肾病 阿霉素 蛋白尿 血管内皮生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]