基于代谢组学分析生草樱桃果园土壤特异代谢物  

Analysis of specific metabolites in the soil of a sweet cherry orchard with grass intercropping based on metabolomics

作　　者：赵柏霞 闫建芳 肖敏 郑玮 李俞涛 夏国芳 张立恒 潘凤荣 ZHAO Baixia;YAN Jianfang;XIAO Min;ZHENG Wei;LI Yutao;XIA Guofang;ZHANG Liheng;PAN Fengrong(Dalian Academy of Agriculture Sciences,Dalian 116036,Liaoning,China;Dalian Minzu University,Dalian 116600,Liaoning,China)

机构地区：[1]大连市农业科学研究院,辽宁大连116036 [2]大连民族大学,辽宁大连116600

出　　处：《草业科学》2024年第1期26-34,共9页Pratacultural Science

基　　金：辽宁省自然科学基金项目(2021-MS-374);辽宁省揭榜挂帅科技公关专项(2021JH1/10400037)。

摘　　要：为了探究甜樱桃(Prunus avium)果园生草对土壤代谢物的影响,本研究以甜樱桃果园行间覆盖鼠茅草(Vulpia myuros)(S)的土壤和行间清耕的土壤(Q)为研究对象,采用超高效液相色谱-四极杆飞行时间质谱(UHPLC-QTOFMS)的代谢组学方法分析土壤中差异代谢产物。结果发现,两组样品中共检测出441种代谢物,其中405种为两者共有,S组样品检测出427种,Q组样品检测出419种。在鉴定出的代谢物中,脂类和类脂分子数量最多,占代谢物总数的33.33%,其次是有机酸及其衍生物,占代谢物总数的23.19%。两组土壤样品筛选得到差异代谢物203种(P<0.01),占共有代谢物的50.12%,其中上调2个,下调201个。差异代谢物主要是脂肪酸及衍生物、氨基酸及衍生物、胺类、萜类、吲哚及衍生物、固醇类及其他物质。KEGG通路富集分析结果显示,有34种差异代谢物富集到了20条代谢通路,其中显著富集(P<0.05)到色氨酸代谢、脂肪酸生物合成、芳香族化合物的降解通路之中。综上,樱桃果园生草对土壤代谢物产生了明显的影响,生草后土壤代谢物种类增加。与清耕对照相比,13个差异代谢物显著下调,分别富集到色氨酸代谢、脂肪酸生物合成、芳香族化合物的降解通路中。其中色氨酸代谢中的邻苯二甲酸下调显著。筛选到的差异代谢物通过参与各种生物过程,进而影响土壤理化性质和微生物的种群。研究结果为樱桃果园生草种植模式影响果园土壤微生态提供理论依据。To explore the effects of intercropping grass on soil metabolites in cherry orchards,the metabolomics approach based on Ultra high performance liquid chromatography-quadrupole time of flight mass spectrometry(UHPLC-QTOF-MS)was used.The differences in metabolites between the soil of living cover crop treatments with Vulpia myuros(S)and clean tillage(Q)were examined.A total of 441 metabolites were detected in the two soil samples.Among these,405 metabolites were shared by both,whereas 427 and 419 metabolites were present in the S and Q samples,respectively.Lipids and lipid molecules were the most abundant,accounting for 33.33%of the identified metabolites.Moreover,organic acids and their derivatives accounted for 23.19%of the total metabolites.In total,203(P<0.01)differential metabolites were identified between the two soil types,accounting for 50.12%of the metabolites common to both soil samples.Among these,two compounds were significantly up-regulated,and 201 compounds were significantly down-regulated.Differential metabolites mainly included fatty acids and their derivatives,amino acids and their derivatives,amines,terpenoids,indoles and their derivatives,steroids,and other substances.KEGG analysis found that 34 differential metabolites were mainly enriched in 20 metabolic pathways,which significantly enriched(P<0.05)tryptophan metabolism,fatty acid biosynthesis,and the aromatic compound degradation pathways.The different metabolites produced affect soil microbial populations and soil physical and chemical properties by participating in various biological processes.In conclusion,intercropping grass in sweet cherry orchards had a marked influence on soil metabolites,and the types of soil metabolites increased compared with the control.Thirteen metabolites were significantly down-regulated,and they enriched the tryptophan metabolism,fatty acid biosynthesis,and aromatic compound degradation pathways.Phthalic acid was significantly down-regulated in tryptophan metabolism.This study provides a theoretical basis for f

关 键 词：土壤微生态 鼠茅草 清耕 行间生草 代谢物 代谢通路 邻苯二甲酸 

分 类 号：S662.5[农业科学—果树学] S154.3[农业科学—园艺学]