芋淀粉合成酶基因家族的鉴定、生物信息学及表达分析  

dentification,bioinformatics and expression analysis of taro starch synthase gene family

作　　者：何芳练 刘莉莉 邱祖杨 董伟清 HE Fang-lian;LIU Li-li;QIU Zu-yang;DONG Wei-qing(Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Lipu Agriculture and Rural Affairs Bureau,Lipu,Guangxi 546600,China)

机构地区：[1]广西壮族自治区农业科学院蔬菜研究所,南宁530007 [2]荔浦市农业农村局,广西荔浦546600

出　　处：《西南农业学报》2024年第1期1-13,共13页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32260762);广西自然科学基金项目(2021GXNSFBA196012);广西农业科学院基本科研业务专项(桂农科2023YM91)。

摘　　要：【目的】鉴定芋淀粉合成酶(CeSS)基因家族,并对其生物信息学及表达模式进行分析,为芋产量、品质和营养性状的遗传改良提供参考。【方法】使用HMMER 3.12b软件以SS基因家族典型保守结构域Glyco_transf_5为模型,鉴定芋基因组中SS基因家族信息,利用生物信息学软件分析其分子结构特征、系统发育关系及顺式作用元件,采用转录组和实时荧光定量PCR技术检测其在正常生长发育条件和干旱胁迫下的转录表达。【结果】在芋基因组中鉴定了6个SS基因(CeSS1、CeSS2、CeSS3⁃1、CeSS3⁃2、CeSS4和CeGBSS1)。6个预测的芋SS基因长度为4980~61347 bp,对应的CDS长度为1275~2895 bp,编码蛋白的氨基酸残基数量为424~964个,分子质量为48067.43~109391.75 Da,理论等电点为5.18~6.11。系统进化分析显示6个芋SS蛋白分布在5个亚家族。基因结构分析显示,6个CeSS基因外显子数量为7~15个。在6个CeSS蛋白中鉴定出10个保守motif,其中7个获得注释。在保守结构域上,CeSS1、CeSS2、CeSS4和CeGBSS1蛋白均含有淀粉合成酶催化结构域(motif 2和motif 5)和糖基转移酶群组1(motif 1和motif 3),而CeSS3⁃1蛋白含有motif 1、motif 3和motif 5,CeSS3⁃2蛋白只含有motif 2。基因启动子区域顺式作用元件分析表明,共预测到73个顺式作用元件,其中36个具有功能注释,除了具有核心元件TATA⁃box和CAAT⁃box外,还涉及大量与光响应、激素响应、胁迫响应及生长发育等相关元件。在正常生长发育条件下,6个CeSS基因在叶片和球茎的整体表达量均高于叶柄和根,其中CeGBSS1基因的表达量远高于其他基因,且在叶片和球茎的相对表达量极显著高于叶柄和根(P<0.01,下同);在球茎不同发育阶段,CeGBSS1基因在所有的发育阶段均有较高表达量,且在30~90 d发育过程中呈上升趋势,其余基因的表达量较低。在干旱胁迫下,6个CeSS基因在叶片的相对表达量较对照组显著(P<0.05)或极显著下�【Objective】The study aimed to identify a family of starch synthase(SS)genes in the taro genome and analyze their bioinformatics and expression patterns to provide a basis for genetic improvement of yield,quality and nutritional traits in taro.【Method】HMMER 3.12b software was used to identify the SS gene family information in the taro genome using the conserved structural domain Glyco_transf_5 as a model,and bioinformatics softwares were used to analyze its molecular structural features,phylogenetic relationships and cis⁃acting elements.Transcriptomic and real⁃time fluorescent quantitative PCR techniques were used to detect its transcriptional expression under normal growth and development conditions and drought stress.【Result】Six SS genes(CeSS1,CeSS2,CeSS3⁃1,CeSS3⁃2,CeSS4 and CeGBSS1)were i⁃dentified in the taro genome.The six predicted taro SS genes ranged in length from 4980 to 61347 bp,corresponding to coding DNA sequence(CDS)lengths from 1275 to 2895 bp,with the number of amino acid residues encoding the protein ranging from 424 to 964,molecu⁃lar weight from 48067.43 to 109391.75 Da,and isoelectric points from 5.18 to 6.11.Phylogenetic analysis revealed that the six taro SS proteins were divided into five subfamilies.Genetic structure analysis showed that the number of exons in the six taro SS genes ranged from 7 to 15.Ten conserved motifs were identified in six CeSS proteins,seven of which were annotated.Conservative structural domain analysis showed that CeSS1,CeSS2,CeSS4 and CeGBSS1 proteins all had starch synthase catalytic domain(motif 2 and motif 5)and glycosyl trans⁃ferase group 1(motif 1 and motif 3),while CeSS3⁃1 protein had motif 1,motif 3 and motif 5 and CeSS3⁃2 protein had only motif 2.Analy⁃sis of the cis⁃acting elements in the promoter region of the gene showed that a total of 73 cis⁃acting elements were predicted,36 of which were functionally annotated and involved a large number of elements related to light response,hormone response,stress response and growt

关 键 词：芋 淀粉合成酶 生物信息学分析 表达分析 

分 类 号：S632.3[农业科学—蔬菜学]