基于SRAP分子标记构建云南旱冬瓜核心种质  

Construction of core collection of Alnus nepalensis in Yunnan based on SRAP molecular markers

作　　者：邹广权 王晓丽[1] 曹现富 李艳 张新洛 曹子林[2] ZOU Guang-quan;WANG Xiao-li;CAO Xian-fu;LI Yan;ZHANG Xin-luo;CAO Zi-lin(Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming 650224,China;College of Ecology and Environment,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学西南山地森林资源保育与利用教育部重点实验室,昆明650224 [2]西南林业大学生态与环境学院,昆明650224

出　　处：《西南农业学报》2024年第1期49-55,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32260385);西南山地森林资源保育与利用教育部重点实验室开放基金项目(KLESWFU⁃201905)。

摘　　要：【目的】通过对比不同抽样比例构建的旱冬瓜种质子集的有效性和代表性,筛选出旱冬瓜核心种质适宜的构建策略。【方法】以旱冬瓜优树同胞子代为试材,设10%、15%、25%、35%、45%和55%共6个抽样比例,采用Nei’s遗传距离和改进的最小距离逐步取样法取样,采用4个遗传多样性指数分析种质子集对原种质的代表性,通过均值t检验和方差F检验分析种质子集与原种质集变异是否同质,利用相关系数分析种质子集与原种质集的相关性,通过遗传距离比较和聚类分析确认核心种质。【结果】25%抽样比例构建的种质子集的多态位点数、多态位点百分率、观测等位基因数和等位基因保留率与原种质集一致,其余4个评价指数皆显著大于原种质集,均值和方差与原种质的相关系数均接近或者等于1,平均遗传距离较原种质提高16.82%,因此认为在25%抽样比例构建的种质子集能很好地代表原种质。【结论】综合考虑核心种质的有效性、实用性、费用和工作量等,认为25%抽样比例构建的旱冬瓜核心种质能充分代表原种质的遗传多样性。【Objective】The suitable construction strategies of core collection of Alnus nepalensis were screened by comparing the effectiveness and representativeness of the proton collection of A.nepalensis species constructed at different sampling ratios.【Method】Using half⁃sib off⁃spring of superior trees of A.nepalensis as test materials,6 sampling ratios were set(10%、15%、25%、35%、45%and 55%),and Nei's genetic distance and the improved minimum distance stepwise sampling method were used to construct the proton collection,and 4 genetic di⁃versity indexes were used to analyze the representativeness of proton collection to the original germplasm.The mean t⁃test and variance F⁃test were used to analyze the homogeneity of the variation between the seed proton set and the original collection,the correlation coefficient was used to analyze the correlation between the seed proton set and the original collection,and the core collection was confirmed by genetic dis⁃tance comparison and cluster analysis.【Result】The number of polymorphic loci,percentage of polymorphic loci,number of observed alleles,and allele retention rate of the germplasm subset constructed with a 25%sampling ratio were consistent with the original germplasm set.The other four evaluation indices were significantly higher than those of the original germplasm set,moreover,the correlation coefficients between mean and variance of the germplasm subset and the original germplasm were close to be equal to 1,with an average genetic distance increase of 16.82%compared to the original germplasm set.Therefore,it was believed that the germplasm subset constructed at a 25%sampling ra⁃tio could effectively represent the original germplasm.【Conclusion】Considering the validity,practicability,cost and workload of the core col⁃lection,it was considered that the core collection of A.nepalensis constructed based on 25%sampling ratio could fully represent the genetic diversity of the original collection.

关 键 词：旱冬瓜 SRAP标记数据 核心种质 

分 类 号：S72[农业科学—林木遗传育种]