Fe_(3)O_(4)@ZIF-8-表面辅助激光解吸/电离质谱法检测美洲大蠊多肽  

Detection of Periplaneta Americana Peptide IPMTAPGL-NH_(2)by Fe_(3)O_(4)@ZIF-8-surface-assisted Laser Desorption/ionization Mass Spectrometry

作　　者：赖心仪 张美铃 王丽丽 林婉真 许惠凤 李琦[1] 胡筱[1] 余丽双[1] LAI Xinyi;ZHANG Meiling;WANG Lili;LIN Wanzhen;XU Huifeng;LI Qi;HU Xiao;YU Lishuang(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122;Fujian Key Laboratory of Integrative Medicine on Geriatrics,Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建省中西医结合老年性疾病重点实验室,福建中医药大学中西医结合研究院,福建福州350122

出　　处：《分析科学学报》2024年第1期29-34,共6页Journal of Analytical Science

基　　金：国家自然科学基金(82074005);福建省科技厅项目(2021J01925)。

摘　　要：本实验在室温下合成了以Zn作为金属中心,2-甲基咪唑作为连接剂的磁性金属有机框架材料Fe_(3)O_(4)@ZIF-8,以Fe_(3)O_(4)@ZIF-8作为固体基质,通过建立Fe_(3)O_(4)@ZIF-8-表面辅助激光解吸/电离质谱(SALDI-MS)法,实现了美洲大蠊小分子多肽IPMTAPGL-NH_(2)、LTAPGL-NH_(2)、SLMTGPGL-NH_(2)、SLHTAPGL-NH_(2)的快速检测。实验优化了基质浓度、检测限和点样方法等条件,考察了该基质对美洲大蠊多肽IPMTAPGL-NH_(2)的质谱信号影响。与传统基质辅助激光解析电离质谱法(MALDI-MS)相比,Fe_(3)O_(4)@ZIF-8-SALDI-MS法检测背景更干净,目标多肽响应更强,检测灵敏度更高。该法可应用于血样中美洲大蠊多肽IPMTAPGL-NH_(2)的快速检测。Peptides from Periplaneta americana are not thermally stable,and the conventional detection methods are often difficult to achieve the desired state.In this experiment,magnetic metal-organic frameworks(MMOFs)Fe_(3)O_(4)@ZIF-8 were synthesized at room temperature,and Fe_(3)O_(4)@ZIF-8 was used as a solid matrix to establish the Fe_(3)O_(4)@ZIF-8-surface-assisted laser desorption/ionization mass spectrometry(SALDI-MS)method,which was applied for the rapid detection of the small molecule peptides from Periplaneta americana,such as IPMTAPGL-NH_(2),LTAPGL-NH_(2),SLMTGPGL-NH_(2)and SLHTAPGL-NH_(2).The matrix effect on mass spectrometric signal of the peptides from Periplaneta americana IPMTAPGL-NH_(2)was investigated by optimizing the matrix concentration,detection limit and spotting method.Compared with matrix-assisted laser desorption/ionization-mass spectrometry(MALDI-MS),Fe_(3)O_(4)@ZIF-8-SALDI-MS method has a cleaner background,stronger response and higher detection sensitivity.This method can be applied to the rapid detection of peptide IPMTAPGL-NH_(2)from Periplaneta americana in blood samples.

关 键 词：磁性金属-有机框架材料 Fe_(3)O_(4)@ZIF-8 美洲大蠊 小分子多肽 

分 类 号：O657.63[理学—分析化学]