泛菌属内生菌YMR3提高花生植株对几种病虫害生物胁迫的抗性研究  

Endophyte Pantoea YMR3 Enhances the Resistance of Peanut Plants to Biological Stresses of Several Diseases and Insect Pests

作　　者：陈容钦 李玲 李晓云 CHEN Rongqin;LI Ling;LI Xiaoyun(Guangdong Provincial Key Laborary of Biotechnology for Plant Development/College of Life Sciences,South China Normal University,Guangzhou 510631,China)

机构地区：[1]华南师范大学生命科学学院/广东省植物发育生物工程重点实验室,广州510631

出　　处：《中国生物防治学报》2024年第1期71-79,共9页Chinese Journal of Biological Control

基　　金：华南师范大学清远研究院开放课题(HQYY2021-013)。

摘　　要：花生在生长过程中常常受到蚜虫、叶螨、黄曲霉和黑曲霉的侵害。如何在花生生长过程中避免或减轻病虫害等生物胁迫的侵害,是解决花生生长发育的重要问题之一。内生菌不仅可以促进花生的生长,还可提高花生对病虫害的抗性。本文探究从粤油7号根系分离出来的内生菌YMR3对抗花生几种病虫害生物胁迫的作用。结果表明,使用OD600nm0.8的YMR3菌液喷施在四叶期花生叶片能够有效降低感染花生蚜虫和叶螨后叶片的损伤程度;使用OD600nm0.8的YMR3菌液注射在四叶期花生顶端叶片能够有效降低黄曲霉和黑曲霉对花生植株或叶片造成的损伤;进一步研究发现,接种YMR3能够提高花生叶片中AhRS1(白藜芦醇的关键合成酶基因)的表达及AhRS1启动子的活性,以及提高花生叶片的白藜芦醇含量;外施100μg/mL白藜芦醇也可明显降低黄曲霉所造成的叶斑比例。以上的结果表明,YMR3可能通过调控花生叶片AhRS1启动子的活性进而调控AhRS1的表达,提高花生叶片白藜芦醇含量以响应黄曲霉对花生叶片的侵害,减轻蚜虫、叶螨、黑曲霉和黄曲霉等对花生叶片的侵害,研究结果为YMR3作为生物农药提供理论基础和依据。During the growth process,peanut plants are often subject to various biological stresses such as aphids,spider mites,Aspergillus flavus,and Aspergillus niger.Avoiding or reducing the damage caused by these pests and diseases is one of the important issues in the development of peanut plants.Endophytic bacteria can not only promote the growth of peanut plants but also enhance their resistance to pests and diseases.This study explored the effect of YMR3,an endophytic bacterium isolated from the roots of Yueyou 7,on the resistance of peanut plants to several pests and diseases.The results showed that spraying peanut leaves at the four-leaf stage with YMR3 bacterial solution at OD600nm 0.8 effectively reduced the damage caused by aphids and spider mites.Injecting the same concentration of YMR3 bacterial solution into the top leaves of peanut plants at the four-leaf stage effectively reduced the damage caused by A.flavus and A.niger to peanut plants or leaves.Further research found that inoculating YMR3 increased the expression of AhRS1(the key synthesis enzyme gene for resveratrol)and the activity of the AhRS1 promoter in peanut leaves,as well as increased the content of resveratrol in peanut leaves.External application of 100μg/mL resveratrol also significantly reduced the proportion of leaf spots caused by A.flavus.These results indicate that YMR3 may regulate the expression of AhRS1 in peanut leaves by regulating the activity of the AhRS1 promoter,increase the content of resveratrol in peanut leaves to respond to the damage caused by A.flavus,and reduce the damage caused by aphids,spider mites,A.niger,and A.flavus to peanut leaves.The research results provide a theoretical basis for the development of YMR3 as a biological pesticide.

关 键 词：花生内生菌 菌株YMR3 抗病虫害胁迫 白藜芦醇 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]