绿原酸对昆虫病原线虫卡森斯氏线虫寿命及抗氧化活性的调控  

Regulation of Longevity and Antioxidant Activity in Entomopathogenic Nematodes Steinernema kraussei by Chlorogenic Acid

作　　者：陈红 李茜童 王兴铎 钱秀娟 CHEN Hong;LI Xitong;WANG Xingduo;QIAN Xiujuan(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070

出　　处：《中国生物防治学报》2024年第1期108-116,共9页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31960559);甘肃省科技计划(21JR7RA819);甘肃农业大学大学生创新创业科研训练计划(202213021)。

摘　　要：为观察绿原酸(CGA)对昆虫病原线虫寿命及抗氧化活性的影响,探究绿原酸对线虫生命活动的调控机制,本文以卡森斯氏线虫作为抗衰老模型,用不同浓度的绿原酸(0、50、100、500、1000μmol/L)处理,探究其对卡森斯氏线虫寿命、抗氧化活性、脂褐素水平、相关基因m RNA表达水平的调控作用,初步探讨绿原酸的潜在作用机理。结果表明,与空白对照组相比,用100μmol/L绿原酸刺激线虫后使处理组最长寿命增加了36.36%,显著抑制体内脂褐素的积累;且该浓度刺激线虫体内的SOD和CAT酶活力,分别提高了1.97倍和2.47倍;此外,q RT-PCR结果显示,100μmol/L CGA喂养线虫后,daf-16、gst-4基因转录水平相比对照分别提高了1.78、3.51,而daf-2基因转录水平呈下降的趋势,降低了0.13。综上可知,绿原酸通过胰岛素/IGF-1信号通路提高昆虫病原线虫卡森斯氏线虫机体的抗氧化能力并延长昆虫病原线虫的寿命。The effects of chlorogenic acid(CGA)on the longevity and antioxidant activity of the entomopathogenic nematodes(EPNs)Steinernema kraussei were investigated through treating S.kraussei with different concentrations of chlorogenic acid(0,50,100,500,1000µmol/L)and then recording S.kraussei longevity,antioxidant activity,lipofuscin levels,and related genes mRNA expression levels,with the aim to explore the potential mechanisms of action of chlorogenic acid.The results showed that the maximum longevity of S.kraussei was increased by 36.36%in the treatment with100µmol/L chlorogenic acid in comparison to the control,significantly inhibited the accumulation of lipofuscin related to aging,but increased SOD and CAT enzyme activities by 1.97 and 2.47 times,respectively.Moreover,qRT-PCR results showed that daf-16 and gst-4 genes transcription levels was increased by 1.78 and 3.51,respectively,in the nematodes fed with 100µmol/L CGA compared with the control.However,the daf-2 gene transcription level showed a downward trend and decreased by 0.13.This study demonstrates that chlorogenic acid enhances the antioxidant activity and prolong the longevity of entomopathogenic nematodes S.kraussei by regulating the insulin/IGF-1 signaling pathway.

关 键 词：绿原酸 昆虫病原线虫 寿命 抗氧化 胰岛素/IGF-1信号通路 

分 类 号：S476[农业科学—农业昆虫与害虫防治]