滋养节杆菌S17的分离鉴定及其对黄瓜促生能力研究  

Isolation and Identification of Arthrobacter pascens S17 and Its Ability to Promote Growth of Cucumber

作　　者：曾先锋 孙钰晨 谢学文[1] 石延霞[1] 柴阿丽[1] 李宝聚[1] 李磊 ZENG Xianfeng;SUN Yuchen;XIE Xuewen;SHI Yanxia;CHAI Ali;LI Baoju;LI Lei(State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Tianjin Agricultural College,Tianjin 300384,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室,北京100081 [2]天津农学院园艺园林学院,天津300384

出　　处：《中国生物防治学报》2024年第1期178-185,共8页Chinese Journal of Biological Control

基　　金：北京设施蔬菜创新团队项目(BAIC01-2023);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业农村部园艺作物生物学与种质创制重点实验室项目;蔬菜生物育种全国重点实验室。

摘　　要：为筛选出具有促生效果的生防细菌,本研究从河北、山东等地采集土样,分离筛选具有高产IAA能力的促生菌株,基于形态学特征、生理生化指标及多基因系统发育树分析,对潜在具有促生能力的菌株进行鉴定。同时测定促生菌株对黄瓜种子出芽及在盆栽试验、田间试验中对黄瓜幼苗的促生效果。结果表明:分离并鉴定出1株对黄瓜幼苗具有显著促生效果的细菌,滋养节杆菌ArthrobacterpascensS17。在DF+培养基中滋养节杆菌S17可大量合成IAA,其合成的IAA含量最高为128.54μg/mL。使用滋养节杆菌S17灌根处理,对黄瓜幼苗株高的增长率达到35.99%、对黄瓜幼苗叶面积的增长率达到26.90%,黄瓜幼苗各项生理指标均显著高于对照。滋生节杆菌S17对黄瓜促生效果显著,具有一定的开发应用前景。In order to screen out the biocontrol bacteria with growth-promoting effect,soil samples were collected from Hebei,Shandong and other places,and the growth-promoting strains with high-yield IAA ability were isolated and screened,and the potential growth-promoting strains with growth-promoting ability were identified based on morphological characteristics,physiological and biochemical indexes and multi-gene phylogenetic tree analysis,and the growth-promoting effects of growth-promoting strains on cucumber seed germination and cucumber seedlings in pot experiments and field experiments were determined.The results showed that one bacterium,Arthrobacter pascens S17,which had a significant growth-promoting effect on cucumber seedlings,was isolated and identified.Arthrobacter pascens S17 could synthesize IAA in large quantities in DF+medium,and the highest IAA content was 128.54μg/mL.The growth rate of cucumber seedling plant height and leaf area of cucumber seedlings reached 35.99%and 26.90%by root irrigation treatment with Arthrobacter pascens S17,and the physiological indexes of cucumber seedlings were significantly higher than those of the control.Arthrobacter pascens S17 has a significant effect on cucumber growth,and has a certain development and application prospect.

关 键 词：滋养节杆菌 鉴定 促生 种子处理 田间试验 

分 类 号：S642.2[农业科学—蔬菜学]