妊娠期糖尿病新型候选生物标志物的筛选效果  

Screening effect of new biomarkers for gestational diabetes

作　　者：王爱绘 丛啸 张新秀 邱海洋 袁悦 倪明 庞乐[1] 黄千千 杨雷 Wang Aihui;Cong Xiao;Zhang Xinxiu;Qiu Haiyang;Yuan Yue;Ni Ming;Pang Le;Huang Qianqian;Yang Lei(Department of Obstetrics and Gynecology,Daqing Fourth Hospital,Daqing 163000,China)

机构地区：[1]大庆市第四医院妇产科,黑龙江大庆163000

出　　处：《实用妇科内分泌电子杂志》2023年第34期41-44,共4页Electronic Journal of Practical Gynecological Endocrinology

摘　　要：目的分析新型候选生物标志物在妊娠期糖尿病(GDM)中的表达水平,并筛选生物标志物,以期为临床早期准确诊断GDM提供指导。方法将77例GDM孕妇归为GDM组,83例正常健康孕妇归为正常组,应用酶联免疫吸附法测定血清标志物蛋白表达水平,应用qRT-PCR法检测人绒毛膜滋养细胞(HTR-8/SVneo)表达水平,比较两组及GDM组不同妊娠时期的血清生物标志物及HTR-8/SVneo表达水平。结果GDM组RAB3B、HNRNPAB、CIRBP、EPPKI、PTEN、Hcy表达水平高于正常组,p-PI3K、p-AKT/AKT、p-mTOR/mTOR表达水平低于正常组(P<0.05);随着妊娠周期不断延长,RAB3B、HNRNPAB、CIRBP、EPPKI、PTEN、Hcy表达水平呈升高趋势,p-PI3K、p-AKT/AKT、p-mTOR/mTOR表达水平呈下降趋势(P<0.05)。结论RAB3B、HNRNPAB、CIRBP、EPPKI、PTEN及p-PI3K、p-AKT/AKT、p-mTOR/mTOR、Hcy可作为GDM新型候选生物标志物,其应用价值有待临床深入研究。Objective To analyze the expression level of new candidate biomarkers in gestational diabetes(GDM)and screen biomarkers in order to provide guidance for early and accurate clinical diagnosis of GDM.Methods 77 pregnant women with GDM were assigned to the GDM group,and 83 healthy pregnant women were included in the normal group.Enzyme linked immunosorbent assay(ELISA)was used to measure the expression levels of serum marker proteins,and qRT PCR was used to detect the expression levels of human chorionic trophoblast cells(HTR-8/SVneo).The serum biomarkers and HTR-8/SVneo expression levels at different pregnancy stages were compared between the two groups and the GDM group.Results The expression levels of RAB3B,HNRNPAB,CIRBP,EPPKI,PTEN,and Hcy in the GDM group were higher than those in the normal group,while the expression levels of p-PI3K,p-AKT/AKT,and p-mTOR/mTOR were lower than those in the normal group(P<0.05);As the pregnancy cycle continues to prolong,the expression levels of RAB3B,HNRNPAB,CIRBP,EPPKI,PTEN,and Hcy show an upward trend,while the expression levels of p-PI3K,p-AKT/AKT,and p-mTOR/mTOR show a downward trend(P<0.05).Conclusion RAB3B,HNRNPAB,CIRBP,EPPKI,PTEN,p-PI3K,p-AKT/AKT,p-mTOR/mTOR,and Hcy can be used as new candidate biomarkers for GDM,and their application value needs further clinical research.

关 键 词：妊娠期糖尿病 血清标志物蛋白表达水平 人绒毛膜滋养细胞 生物标志物 应用价值 

分 类 号：R714.256[医药卫生—妇产科学]