泰山磐石散对复发性流产小鼠母胎界面TGF-β、VEGF、PIGF影响  

Effects of Taishan Panshi Powder(泰山磐石散)on TGF-β,VEGF and PIGF at Maternal Fetal Interface of Mice with Recurrent Abortion

作　　者：邱林 何飞 许春艳[2] 孙晶[2] QIU Lin;HE Fei;XU Chunyan;SUN Jing(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2024年第3期18-21,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：沈阳市科技计划项目(21-174-9-06);辽宁中医药大学附属医院育苗工程项目(YM202117)。

摘　　要：目的 观察泰山磐石散对复发性流产(RSA)小鼠母胎界面血管生成相关调控因子[转化生长因子-β(TGF-β)、血管内皮生长因子(VEGF)、胎盘生长因子(PIGF)]、胎盘蜕膜组织病理学形态的影响,并探讨其降低流产率的作用机制。方法 构建RSA小鼠模型32只,将造模成功的小鼠随机分为4组:模型组及泰山磐石散高、中、低剂量组,每组各8只;构建正常妊娠小鼠模型8只,作为空白组。自妊娠第1天,分别予相应药物干预,14 d后处死孕鼠,收集样本并计算小鼠流产率;采用苏木精-伊红(HE)染色对小鼠胎盘蜕膜组织病理形态变化进行观察;采用ELISA法检测小鼠血清中TGF-β、VEGF、PIGF含量。结果 模型组胎盘及蜕膜组织细胞形态不规则,排列紊乱,血管壁不完整且血管数量明显减少;经泰山磐石散方药干预后,各中药组小鼠胎盘及蜕膜组织病理形态均得到明显改善。与空白组相比,模型组小鼠流产率明显增加(P<0.05),血清中TGF-β、VEGF、PIGF含量显著降低(P<0.05);与模型组比较,泰山磐石散高、中剂量组均可有效降低小鼠流产率(P<0.05),且显著升高血清中TGF-β、VEGF、PIGF含量(P<0.05),其中泰山磐石散高剂量组降低流产率、升高血管生成相关调控因子情况尤为显著。结论 泰山磐石散通过上调TGF-β、VEGF、PIGF含量,参与复发性流产小鼠母胎界面的血管重塑过程,改善血管壁通透性,维持血流供应,从而改善胎盘蜕膜组织病理形态,发挥防治流产作用,提高RSA小鼠胚胎存活率。Objective To investigate the effect of Taishan Panshi Powder(泰山磐石散)on the angiogenic regulatory factors(TGF-β,VEGF,PIGF)and the histopathological morphology of decidual plate at the maternal and fetal interface in recurrent miscarriage(RSA)mice,and to investigate the mechanisms of its effect on the reduction in the rate of miscarriage.Methods For the constructed RSA mouse model 32,mice were randomly divided into four groups:the model group,the low-dose group,and the high-dose,medium-dose group,each of eight mice;for the constructed normal pregnancy model,eight mice were used as the blank group.From the first day of pregnancy,mice were sacrificed after 14 days of drug treatment,samples were collected,and miscarriage rates were calculated;hematoxylin and eosin(HE)staining was performed to observe histopathological morphology changes in decidual platelets;and serum TGF-β,VEGF,and PIGF were measured by ELISA.Results The model group had irregular morphology,disordered arrangement,incomplete blood vessel wall and significantly reduced number of blood vessels.After the intervention of Taishan Panshi Powder Medicine,the histopathological morphology of placenta and decidua in all traditional Chinese medicine groups of mice were significantly improved.Compared with the blank group,the miscarriage rate of mice in the model group increased significantly(P<0.05),and the contents of TGF-β,VEGF and PIGF in serum decreased significantly(P<0.05).Compared with the model group,the high-dose group and medium-dose group of Taishan Panshi Powder effectively reduced the abortion rate(P<0.05)in mice,and significantly increased the contents of TGF-β,VEGF and PIGF in serum(P<0.05),among which the high-dose group of Taishan Panshi Powder reduced the miscarriage rate and increased the associated angiogenic factors.Conclusion By upregulating the contents of TGF-β,VEGF and PIGF,Taishan Panshi Powder participated in the vascular remodeling process of the mother-fetal interface of recurrent aborted mice,improved the permeability

关 键 词：泰山磐石散 复发性流产 母胎界面 转化生长因子-Β 血管内皮生长因子 胎盘生长因子 

分 类 号：R271.4[医药卫生—中医妇科学] R289.5[医药卫生—中医学]