羚羊感冒口服液多重药理作用研究  

Multiple pharmacological effects of Lingyang Ganmao Oral Liquid

作　　者：孙绮悦 赵荣华[1] 郭姗姗[1] 包蕾[1] 耿子涵 李舒冉 徐英莉 张敬升 崔晓兰[1] 孙静[1] SUN Qiyue;ZHAO Ronghua;GUO Shanshan;BAO Lei;GENG Zihan;LI Shuran;XU Yingli;ZHANG Jingsheng;CUI Xiaolan;SUN Jing(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100029,China)

机构地区：[1]中国中医科学院中药研究所,北京100029

出　　处：《中国药物警戒》2024年第2期127-131,共5页Chinese Journal of Pharmacovigilance

基　　金：中国中医科学院科技创新工程项目(CI2021B015);国家重点研发计划(2020YFE0205100)。

摘　　要：目的在动物水平对羚羊感冒口服液多重药理作用进行评价,为其临床应用提供理论依据和数据支持。方法依据羚羊感冒口服液的功能主治及临床适应证,建立6种动物模型:流感病毒FM1株及肺炎链球菌感染小鼠致肺炎模型,冰醋酸致小鼠腹腔毛细血管通透性模型及扭体模型,氨水致小鼠咳嗽模型及小鼠气管酚红排泌模型。通过观察肺指数、肺指数抑制率、伊文思蓝OD值、扭体次数,咳嗽次数及酚红排泌量等药效指标,评价羚羊感冒口服液的抗病毒、抗菌、抗炎、止痛、止咳、化痰作用。结果羚羊感冒口服液20 mL·kg^(-1)·d^(-1)剂量组可显著抑制流感病毒FM1引起的肺指数增高,明显增加小鼠气管酚红排泌量;肺炎链球菌感染小鼠的肺指数升高及腹腔毛细血管通透性增强,羚羊感冒口服液20、15、10 mL·kg^(-1)·d^(-1)3个剂量组对咳嗽有明显抑制作用,可显著减少氨水引起的小鼠咳嗽次数,延长咳嗽潜伏期;20、15 mL·kg^(-1)·d^(-1)2个剂量组均可显著减少醋酸致小鼠疼痛的扭体次数。结论不同剂量组羚羊感冒口服液作用程度不同且具有多重药理作用,包括抗病毒、抗菌、抗炎、止痛、止咳、化痰作用等。Objective To evaluate the pharmacological effects of Lingyang Ganmao Oral Liquid at the animal level,and to provide theoretical basis and data to support clinical precision medicine.Methods Six animal models were established according to the functional indications and clinical indications of Lingyang Ganmao Oral Liquid,the pneumonia model of mice infected by influenza virus FM1 strain and Streptococcus pneumoniae,the peritoneal capillary permeability model and body twisting model of mice induced by ice acetic acid,the cough model of mice induced by ammonia water,the trachea phenol red excretion model of mice.The antiviral,antibacterial,anti-inflammatory,analgesic,relieving cough and eliminating phlegm effects of Lingyang Ganmao Oral Liquid were evaluated by observing pulmonary index,inhibitory rate of lung index,OD value of Evanslan,writhing times,coughing times and phenol red excretion.Results Lingyang Ganmao Oral Liquid 20 mL·kg^(-1)·d^(-1)can significantly inhibit the increase of lung index caused by influenza virus FM1,and significantly increase the secretion of phenol red in the tracheal of mice.The lung index and peritoneal capillary permeability of Streptococcus pneumoniae infected mice were increased.Lingyang Ganmao Oral Liquid 20,15,10 mL·kg^(-1)·d^(-1)three dose groups had significant inhibitory effect on both of them,which could significantly reduce the frequency of cough caused by ammonia water in mice and prolong the incubation period of cough.Both 20 and 15 mL·kg^(-1)·d^(-1)dose groups could significantly reduce the number of writhe caused by acetic acid in mice.Conclusion Lingyang Ganmao Oral Liquid of different doses has different degrees of action and multiple pharmacological effects,including antiviral,antibacterial,anti-inflammatory,analgesic,cough and expectorant.

关 键 词：羚羊感冒口服液 抗病毒 抗菌 抗炎 止咳 化痰 肺指数 酚红排泌量 小鼠 

分 类 号：R965.1[医药卫生—药理学] R994.11[医药卫生—药学]