拉莫三嗪分子印迹膜的制备及其吸附性能研究  

Preparation and Recognition Features of Molecularly Imprinted Polymer Membrane for Lamotrigine in Plasma

作　　者：陆东裕 尤宇欣 赵燕琳 蒋函 张迎 杜岩 汤道权 LU Dong-Yu;YOU Yu-Xin;ZHAO Yan-Lin;JIANG Han;ZHANG Ying;DU Yan;TANG Dao-Quan(Department of Pharmacy,Suining Branch of the Hospital Affiliated to Xuzhou Medical University,Suining 221202,China;Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy,Xuzhou Medical University,Xuzhou 221004,China)

机构地区：[1]徐州医科大学附属医院睢宁分院药学部,睢宁221202 [2]徐州医科大学,江苏省新药研究与临床药学重点实验室,徐州221004

出　　处：《分析化学》2024年第1期80-92,共13页Chinese Journal of Analytical Chemistry

基　　金：江苏省自然科学基金项目(Nos.BK20181147,BE2019640)资助。

摘　　要：利用表面分子印迹技术,以聚偏氟乙烯(Polyvinylidene fluoride,PVDF)膜为载体、拉莫三嗪(Lamotrigine,LTG)为模板、甲基丙烯酸甲酯为功能单体、甲基丙烯酸乙二醇酯为交联剂、偶氮二异丁腈为引发剂、乙腈-二甲基酰胺(1∶1.5,V/V)为致孔剂,制备了可用于高选择性快速分离血浆中LTG的分子印迹膜(Molecularly imprinted polymer membranes,MIPMs)。利用扫描电子显微镜、红外光谱仪、比表面积检测仪、X射线光电子能谱分析及热重分析对MIPMs进行了表征,通过动力学吸附、等温吸附、选择性吸附、吸附-解吸附和重复利用性实验探究了MIPMs的吸附性能。结果表明,PVDF表面成功包裹了LTG分子印迹聚合物,并且材料颗粒均匀。所制备的MIPMs对LTG的吸附容量为3.77 mg/g、印迹因子为8.97,并具有较快的传质速率(30 min)以及良好的重复利用性(6次重复循环使用后吸附效率为86.66%),对血浆中LTG的提取回收率为86.54%~90.48%,相对标准偏差(RSD)为1.51%~3.15%(n=5)。本研究制备的LTG-MIPMs具有操作简单、吸附速度快、吸附容量大、选择性高以及环境友好等优点,适用于血浆中LTG的快速选择性分离提取。The molecularly imprinted polymers membranes(MIPMs)were prepared for selective adsorption of lamotrigine(LTG)in plasma by surface molecular imprinting technology with polyvinylidenefluoride(PVDF)membranes as supporter,lamotrigine as template molecule,methyl methacrylate as functional monomer,ethylene glycol dimethacrylate as cross-linking agent,azodiisobutyronitrile as initiator and acetonitrile-dimethylformamide(1∶1.5,V/V)as pore-forming agent.The prepared MIPMs were characterized by scanning electron microscope,Fourier transform infrared spectroscopy,Brunaner-emmet-teller measurements,X-ray photoelectron spectroscopy,and thermogravimetric analysis.The adsorption properties of the materials were investigated by kinetic adsorption,isothermal adsorption,selective adsorption,adsorption-desorption and reusability experiments.The results showed that the imprinted layer of LTG was successfully coated on the surface of PVDF,and the materials had uniform particle size.The adsorption capacity and imprinting factor of the MIPMs towards LTG were 3.77 mg/g and 8.97,respectively.The nanomaterials showed fast mass transfer rate(30 min)and good reusability(the adsorption efficiency was 86.66%after 6 cycles),and could be used for the adsorption of LTG in plasma with low matrix interference,recoveries of 86.54%–90.48%and RSD of 1.51%–3.15%(n=5).The proposed LTG MIPMs were demonstrated to be simple and environment friendly,and had high selectivity in rapid separation and extraction of LTG in plasma.

关 键 词：分子印迹聚合物膜 拉莫三嗪 选择性吸附 血浆 

分 类 号：R969[医药卫生—药理学] TB383.2[医药卫生—药学] O647.3[一般工业技术—材料科学与工程]