香薷挥发油对吴茱萸有效成分透皮吸收的影响  

Study on the Effect of Moslae Herba Oil on the Percutaneous Absorption of Active Ingredient of Evodia Rutaecarpa in vitro

作　　者：李秋娟 梁亚芳 舒娟 贾聪玲 刘潺潺 吴啟南[1,2,3] LI Qiujuan;LIANG Yafang;SHU Juan;JIA Congling;LIU Chanchan;WU Qinan(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of TCM Resource Industrialization Process,Nanjing 210023,China;National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023 [3]中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《南京中医药大学学报》2024年第1期26-36,共11页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81903756);国家中医药管理局项目道地药材生态种植及质量保障项目(2022);30种中药饮片产地加工与炮制生产一体化关键技术规范研究(201507002)。

摘　　要：目的考察香薷挥发油对吴茱萸药效成分的促渗作用以及其促透皮吸收的能力。方法采用扩散池和大鼠离体皮肤进行体外透皮实验,以吴茱萸中多种成分作为指标,HPLC测定计算各成分累积透过量。HE染色、免疫组化染色、ATR-FTIR观察皮肤及角质层的变化,GC-MS测定挥发油给药后皮内贮存挥发油成分。结果香薷挥发油对吴茱萸中多种药效成分均具有促渗作用,与阳性药氮酮相比,香薷挥发油对金丝桃苷、吴茱萸碱、吴茱萸次碱的累积透过量更大。且香薷挥发油中倍半萜成分及含量占比最大的麝香草酚更易在皮肤中贮存,可以扰乱角质层脂质结构,促进药物的渗透。结论为透皮吸收促进剂的选择提供参考。OBJECTIVE To investigate whether Moslae herba oil has penetration enhancing effect on the active components of Evodia rutaecarpa and its ability to promote transdermal absorption.METHODS In vitro transdermal experiments were performed using the diffusion pool and the isolated skin of rats.Using various components of Evodia rutaecarpa as indicators,HPLC was determined to calculate the umulative penetration amount of each component.Changes in the skin and cuticle layers were observed by HE staining,immunohistochemical staining,and ATR-FTIR.GC-MS was used to measure Moslae herba oil components after administration.RESULTS Moslae herba oil showed the penetration-enhancing effect on various components in Evodia rutaecarpa.Compared with the postive drug nitrogenone,the cumulative penetration amounts of hyperoside,evodiamine and rutaecarpine were greater.In addition,the sesquiterpene composition and thymol in the Moslae herba oil were more likely to be stored in the skin,which could disturb the lipid structure of the cuticle layer and promote the penetration of drugs.CONCLUSION The results provide reference for the selection of transdermal absorption agent.

关 键 词：香薷挥发油 吴茱萸 透皮吸收促进剂 

分 类 号：R284[医药卫生—中药学]