数字化技术构建SD大鼠颞下颌关节骨关节炎动物模型  

Constructing an animal model of temporomandibular joint osteoarthritis in Sprague-Dawley rats by digital technology

作　　者：刘鹏慧 吴凡 王泽杰 吴高义 周立波[1] Liu Penghui;Wu Fan;Wang Zejie;Wu Gaoyi;Zhou Libo(Experimental Center for Stomatology Engineering&Heilongjiang Key Lab of Oral Biomedicine Materials and Clinical Application,Jiamusi University Affiliated Stomatological Hospital,Jiamusi University,Jiamusi 154000,Heilongjiang Province,China)

机构地区：[1]佳木斯大学附属口腔医院,佳木斯大学口腔医学工程实验中心,黑龙江省口腔生物医学材料与临床应用重点实验室,黑龙江省佳木斯市154000

出　　处：《中国组织工程研究》2024年第35期5642-5648,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(61771290,61871393),项目负责人:吴高义;黑龙江省自然科学基金项目(LH2021H108),项目负责人:周立波;黑龙江省教育厅青年人才创新计划项目(UNPYSCT-2020057),项目负责人:周立波;佳木斯大学青年创新人才培养计划项目(JMSUQP2020020),项目负责人:周立波。

摘　　要：背景:颞下颌关节骨关节炎是一种发病率很高的口腔常见病,而颞下颌关节骨关节炎早期隐匿不易被发现,临床上病理标本获取难度较高,难以开展相关研究。将数字化3D打印技术应用到颞下颌关节骨关节炎动物模型上来增加动物模型的一致性,从而促进颞下颌关节骨关节炎疾病的研究。目的:利用新型数字技术建立标准化的颞下颌关节骨关节炎大鼠模型。方法:将30只SD雌性大鼠根据单侧前牙反牙合建模方法的不同,随机分为3组,传统模型组、数字化模型组和对照组,每组10只。于建模后4,8周采集髁突软骨标本,体式显微镜观察表观形态,苏木精-伊红染色、番红O/固绿染色观察病理形态,ELISA观察白细胞介素1β、肿瘤坏死因子α的表达变化,免疫组织化学染色观察蛋白多糖、Ⅱ型胶原及基质金属蛋白酶13的表达变化。结果与结论:(1)数字化模型组和传统模型组观察到不同程度的退行性病变。干预后第1周,模型组大鼠体质量均呈下降趋势,随后呈增长趋势,且显著低于对照组。(2)体式显微镜观察结果显示,建模后4,8周,数字化模型组表面的变形及缺损程度明显高于传统模型组。(3)两个时间点,数字化模型组和传统模型组的国际骨关节炎研究学会(Osteoarthritis Research Society International,OARSI)评分均高于对照组,数字化模型组高于传统模型组(P<0.05)。(4)组织病理学观察显示,建模后4,8周,两个模型组的改良Mankin评分和OARSI评分均显著高于同龄对照组(P<0.05)。(5)免疫组织化学染色结果显示,建模后4,8周,与同龄对照组相比,蛋白多糖及Ⅱ型胶原在传统模型组与数字化模型组两个时间点表达降低,基质金属蛋白酶13表达增强(P<0.05)。(6)ELISA结果显示,传统和数字化模型组大鼠8周时炎性因子白细胞介素1β和肿瘤坏死因子α的表达水平均高于同龄对照组,数字化模型组高于传统模型组(P<0.05)。(7)BACKGROUND:Temporomandibular joint osteoarthritis is a common oral disease with a high incidence.However,temporomandibular joint osteoarthritis is not easy to be detected in the early stage,and it is difficult to obtain clinical pathological specimens,so it is difficult to carry out related research.The application of digital 3D printing technology to animal models of Temporomandibular joint osteoarthritis increases the consistency of the animal models,thus promoting the study of temporomandibular joint osteoarthritis.OBJECTIVE:To establish a standardized animal model of temporomandibular joint osteoarthritis using novel digital technology.METHODS:According to the different modeling methods of unilateral anterior crossbite,30 female Sprague-Dawley rats were randomly divided into traditional model group,digital model group,and control group(n=10 per group).Cartilage specimens of the condyles were collected at 4 and 8 weeks after modeling.The apparent morphology was observed by stereoscopic microscope.The pathological morphology was observed by hematoxylin-eosin staining and Safranin O/fast green staining.Changes in the expression of interleukin-1βand tumor necrosis factor-αwere observed by ELISA,and changes in the expression of aggrecan,type II collagen and matrix metalloproteinase-13 were observed by immunohistochemical staining.RESULTS AND CONCLUSION:Different degrees of degeneration were observed in the digital and traditional model groups.The body mass of rats in both the model groups decreased during the 1st week after intervention and subsequently demonstrated growth trend and were significantly lower than that in the control group.The results of stereoscopic microscope showed that at 4 and 8 weeks after modeling,the deformation and defect degree of the digital model group was significantly higher than that of the traditional model group.At these two time points,the Osteoarthritis Research Society International scores of the digital model group and the traditional model group were higher than those of the

关 键 词：颞下颌关节 骨关节炎 数字化技术 3D打印 动物模型 单侧前牙反牙合 咬合 髁突软骨 退行性病变 高精度 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364