环状RNA通过细胞内机制参与骨关节炎的发病  

Circular RNAs are involved in the pathogenesis of osteoarthritis through intracellular mechanisms

作　　者：周丽君 张克远 王茜[1,3] 俞丽 徐飞虎[1,3] 丁红 马海蓉 Zhou Lijun;Zhang Keyuan;Wang Xi;Yu Li;Xu Feihu;Ding Hong;Ma Hairong(Institute of Clinical Medical Research,Department of Comprehensive Cardiology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Department of Nutrition and Food Hygiene,School of Public Health,School of Pharmaceutical Science,Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Orthopedic Center,Department of Comprehensive Cardiology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Cardiac Center,Department of Comprehensive Cardiology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;Department of Pharmacology,School of Pharmaceutical Science,Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院临床医学研究院,新疆维吾尔自治区乌鲁木齐市830011 [2]新疆医科大学公共卫生学院营养与食品卫生学教研室,新疆维吾尔自治区乌鲁木齐市830011 [3]新疆医科大学第一附属医院骨科中心,新疆维吾尔自治区乌鲁木齐市830011 [4]新疆医科大学第一附属医院心脏中心,综合心脏内科,新疆维吾尔自治区乌鲁木齐市830011 [5]新疆医科大学药学院药理教研室,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2024年第35期5716-5722,共7页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金重点项目(2021D01D21),项目负责人:马海蓉;国家自然科学基金项目(82060411),项目负责人:马海蓉。

摘　　要：背景:目前并没有药物可以完全治愈骨关节炎,且其发病机制尚不清楚。环状RNA(circRNA)在骨关节炎患者中的差异表达与骨关节炎的各种病理过程密切相关,circRNA在软骨细胞稳态、细胞外基质形成和炎症反应等生理病理过程中发挥重要作用。目的:综述circRNA对骨关节炎相关病理因素的影响,以及骨关节炎中circRNA的种类和水平。方法:检索中国知网、万方数据库、维普数据库、PubMed、Medline、Web of Science和Elsevier数据库,以“osteoarthritis,circular RNA,non-coding RNA,synovial tissue,chondrocytes”为英文检索词,以“骨关节炎,环状RNA,非编码RNA,滑膜组织,软骨细胞”为中文检索词,检索1976年至2023年8月前发表的所有相关文献,并对其进行筛选、归纳、分析、总结,最后纳入69篇文献进行综述。结果与结论:(1)circRNA是在真核细胞中广泛发现的非编码RNA,具有共价闭合的连续环结构,没有5′帽子结构和3′poly A尾巴,参与多基因、多靶点调节网络,不会被核酸外切酶(RNase R)降解;(2)circRNA具有高丰度、高度保守性和稳定性、细胞和组织特异性等特点;(3)circRNA具有充当miRNA的分子海绵、调节线性RNA转录及RNA剪切、与RNA结合蛋白质相互作用以及翻译蛋白质等生物学功能;(4)circRNA调控软骨细胞的凋亡与增殖、软骨细胞外基质的降解以及炎症反应等生理病理进程;(5)circRNA有望成为骨关节炎临床诊断及治疗预后的生物标志物和潜在治疗靶点,未来可能会成为骨关节炎临床治疗的新策略。BACKGROUND:Currently,there is no drug that can completely cure osteoarthritis and its pathogenesis is still unclear.Circular RNAs(circRNAs)are differentially expressed in patients with osteoarthritis and are closely associated with various pathological processes in osteoarthritis.circRNAs play an important role in various physiological and pathological processes,such as chondrocyte homeostasis,extracellular matrix formation,and inflammatory response.OBJECTIVE:To mainly review the effects of circRNAs on pathological factors related to osteoarthritis,as well as the types and expression levels of circRNAs in osteoarthritis.METHODS:Related articles published from 1976 to August 2023 were retrieved from CNKI,WanFang,VIP,PubMed,Medline,Web of Science and Elsevier databases.The keywords were“osteoarthritis,circular RNA,non-coding RNA,synovial tissue,chondrocytes”in Chinese and English,respectively.All the relevant articles were screened,summarized,analyzed,and finally 69 papers were included in the review.RESULTS AND CONCLUSION:circRNAs are non-coding RNAs widely found in eukaryotic cells,with covalently closed continuous loop structure,but with no 5′hat structure and 3′poly A tail,which are involved in multi-gene and multi-target regulatory networks and cannot be degraded by nucleic acid exonucleases(RNase R).circRNAs have a high abundance,high conservativeness and stability,and cell and tissue specificity.circRNAs have biological functions such as acting as molecular sponges for miRNAs,regulating linear RNA transcription and RNA shearing,interacting with RNA-bound proteins,and translating proteins.circRNAs regulate chondrocyte apoptosis and proliferation,degradation of cartilage extracellular matrix,and inflammation and other physiopathologic processes.circRNAs are expected to become biomarkers and potential therapeutic targets for clinical diagnosis and prognosis of osteoarthritis,and may become a new strategy for clinical treatment of osteoarthritis in the future.

关 键 词：circRNA 骨关节炎 关节软骨 软骨细胞 治疗靶点 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R496