Clean-DM1, a Korean Polyherbal Formula, Improves High Fat Diet-Induced Diabetic Symptoms in Mice by Regulating IRS/PI3K/AKT and AMPK Expressionsin Pancreas and Liver Tissues  

作　　者：Piao Wang Yi Liu Seok Yong Kang Chenzi Lyu Xiang Han Tianjun Ho Kyung Jae Lee Xianglong Meng Yong-Ki Park Hyo Won Jung 

机构地区：[1]Department of Herbology,College of Korean Medicine,Dongguk University,Gyeongju 38066,Korea [2]College of Pharmacy&Graduate School of Pharmaceutical Sciences,Ewha Womans University,Seoul 03760,Korea [3]Korean Medicine R&D Center,Dongguk University,Gyeongju 38066,Korea [4]Shanxi Key Laboratory of Traditional Herbal Medicines Processing,Shanxi University of Chinese Medicine,Jinzhong(030619),ShanxiProvince,China

出　　处：《Chinese Journal of Integrative Medicine》2024年第2期125-134,共10页中国结合医学杂志（英文版）

基　　金：Supported by Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI),the Ministry of Health&Welfare,Republic of Korea (No. HF20C0121);Shanxi Key Laboratory of Tradition Herbal Medicines Processing (No. 20210901);the Innovation Team of Shanxi University of Chinese Medicine (No. 2022TD1014)

摘　　要：Objective:To investigate the effects of Clean-DM1(C-DM1),a polyherbal formulation of Radix Scrophulariae,Radix Astragali,Rhizoma Atractylodis,and Radix Salviae Miltiorrhizae,on high-fat diet(HFD)-induced diabetes mice.Methods:The information about active components of C-DM1 extract and molecular mechanism was obtained from network pharmacology analysis.Main compounds of C-DM1 extract by high performance liquid chromatography-mass spectrometry(HPLC-MS)analysis were conducted for quality control.For in vivo study,mice were induced diabetes by HFD for 12 weeks.The mice in the normal group(Nor)were maintained with a regular diet and treated with saline by gavage.The HFD model mice were randomly divided into 3 groups,including a HFD diabetic model group,a C-DM1 extract-administered group(C-DM1,500 mg/kg),and metformin-administered groups(Met,500 mg/kg),8 mice in each group.Food intake,body weight(BW),and fasting blood glucose(FBG)levels were recorded weekly for 4 weeks.After 4 weeks of treatment,alanine aminotransferase(ALT),aspartate aminotransferase(AST),blood glucose,low-density lipoprotein cholesterol(LDL-C)were determined using an automated clinical chemistry analyzer,and homeostatic model for assessing insulin resistance(HOMA-IR)levels and oral glucose tolerance test(OGTT)were detected.The histopathological changes of liver and pancreatic tissues were observed by hematoxylin-eosin staining.Insulin receptor substrate(IRS)/phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)and adenosine 5'-monophosphate-activated protein kinase(AMPK)expressions in liver and pancreas tissues were detected by Western blot analysis.Results:HPLC-MS identified dihydroisotanshinone,dihydroisotanshinone I,cryptotanshinone,harpagoside,and atractyloside A in C-DM1 extract.The administration of C-DM1 extract significantly decreased body weight,calorie intake,and the levels of blood glucose and insulin in the diabetic mice(P<0.05 or P<0.01).The C-DM1 extract administration improved the impaired glucose tolerance and insulin resistance

关 键 词：high-fat diet type 2 diabetes mellitus herbal formulation insulin receptor substrate/phosphatidylinositol 3 kinase/protein kinase B adenosine 5'-monophosphate-activated protein kinase network pharmacology high performance liquid chromatography-mass spectrometry analysis 

分 类 号：R285.5[医药卫生—中药学]